VEGF Antibody (JH121) recommended for detection of all forms of VEGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VEGF Antibody (JH121): sc-57496

VEGF Antibody (JH121) is rated 4.5 out of 5 by 8.
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Datasheets

    • VEGF Antibody (JH121) is a mouse monoclonal IgG1 (kappa light chain) provided at 50 µg/0.5 ml
    • raised against full length VEGF of human origin
    • recommended for detection of all forms of VEGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See VEGF (C-1): sc-7269 for VEGF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VEGF (JH121): sc-57496.
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Buenas tardes, ustedes me pueden dar la fecha de caducidad de los siguientes anticuerpos? VEGF sc-57496 (JH121) Lote C2813 E-cadherin sc-8426 (G-10) Lote F3011 HIF-1alfa sc-13515 (28b) Lote F2513

Asked by: Mashenka
Gracias por su consulta. No caracterizamos fechas de vencimiento para nuestros anticuerpos. Los cubrimos por garantía por un año a partir de la fecha en que se reciben. El anticuerpo puede ser estable más allá de esta fecha, pero ya no lo garantizamos. Thank you for your inquiry. We do not characterize expiration dates for our antibodies. We cover them by warranty for one year from the date they are received. The antibody may be stable beyond this date, but we will no longer guarantee it.
Answered by: Tech Service 3
Date published: 2017-05-09

Is VEGF (JH121): sc-57496 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using VEGF (C-1): sc-7269, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/vegf-antibody-c-1)
Answered by: Technical Support 10
Date published: 2017-01-12

Can VEGF (JH121): sc-57496 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, VEGF (JH121): sc-57496 monoclonal antibody is recommended for with paraffin-embedded sections. We suggest performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by from Works very well Used at a dilution of 1:500 and loaded 30ug. saw very dark intense bands. The dilution can be increased upto 1:1000, easily
Date published: 2019-09-06
Rated 3 out of 5 by from IHC detection only in rat INS-1 xenografts Clean DAB-based detection in rat INS-1 xenografts, but no bands detected in lysates by Western blot. Antibody sc-53462 was superior in both methods.
Date published: 2019-05-22
Rated 5 out of 5 by from band at 21kda The antibody was cited by paper "Pleiotrophin is a potential colorectal cancer prognostic factor that promotes VEGF expression and induces angiogenesis in colorectal cancer"we use the same sample and detect by WB, the result shows specific band at 21kda.
Date published: 2017-06-24
Rated 5 out of 5 by from Good results in WB Good specificity and strong signal strength in 293T whole cell lysates
Date published: 2017-05-10
Rated 5 out of 5 by from Great WB and IHC data Strong western blot results; also very nice IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by from Good VEGF antibody Good data for staining and WB. Antibody recommended for mouse, rat, and human VEGF detection.
Date published: 2017-01-27
Rated 3 out of 5 by from Faint detection of upregulation of a single Faint detection of upregulation of a single band in WB with transfected HEK293T cell lysates -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Works very well in IHC with human gastric Works very well in IHC with human gastric carcinomas -SCBT Publication Review
Date published: 2015-01-06
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