Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VDUP1 CRISPR/Cas9 KO Plasmid (h): sc-400664
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VDUP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VDUP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VDUP1 Antibody (D-2): sc-271237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VDUP1 CRISPR/Cas9 KO Plasmid (h) | sc-400664 | 20 µg | $397.00 |
Overview
Thioredoxin interacting protein (TXNIP), also known as VDUP1, is a redox-sensitive regulator that binds thioredoxin to modulate cellular oxidative stress responses and redox homeostasis. TXNIP links nutrient and stress signaling to metabolic control by influencing glucose uptake, mitochondrial function, and inflammatory outputs, and it participates in pathways such as AMPK signaling, ROS handling, and NLRP3 inflammasome-associated processes. Altered TXNIP/VDUP1 expression has been associated with metabolic dysregulation, endothelial dysfunction, and inflammatory states, and it is frequently studied in contexts of diabetes-related complications, cardiovascular biology, and cancer cell metabolism. As a stress-inducible gene, TXNIP is also used as a readout and driver of transcriptional programs downstream of ER stress and oxidative signaling.
VDUP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TXNIP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TXNIP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TXNIP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VDUP1 protein expression.
This CRISPR knockout system enables efficient generation of TXNIP-deficient cell models for investigation of VDUP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TXNIP exon(s) critical for VDUP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TXNIP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VDUP1 CRISPR/Cas9 KO Plasmid (h) and VDUP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TXNIP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VDUP1 HDR Plasmid (h) and VDUP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TXNIP homology arms to support homology-directed repair at defined TXNIP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.