VDUP1 Antibody (B-2): sc-166234

VDUP1 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects VDUP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VDUP1 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The gene encoding vitamin D3 upregulated protein 1 (VDUP1) is notably upregulated by 1,25(OH)2D3 in response to various cellular stresses, including reactive oxygen species, ultraviolet radiation, and heat shock, with the transcription factor heat shock factor potentially playing a role in this regulation. VDUP1 serves as a natural antagonist of thioredoxin, contributing to tumor-suppressive activity by inducing cell cycle arrest at the G0/G1 phase, which is crucial for preventing uncontrolled cell proliferation. Additionally, VDUP1 is essential for CD122 expression and natural killer (NK) cell maturation, although VDUP1′s influence on T and B cell development is relatively minimal. The human VDUP1 gene is located on chromosome 1q21, and VDUP1 exhibits ubiquitous expression across various tissues, predominantly localizing to the cytoplasm. Given VDUP1′s significant roles in cellular stress responses and immune regulation, VDUP1 may represent a promising therapeutic target for melanoma.

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VDUP1 Antibody (B-2) References:

1. Identification of thioredoxin-binding protein-2/vitamin D(3) up-regulated protein 1 as a negative regulator of thioredoxin function and expression.  |  Nishiyama, A., et al. 1999. J Biol Chem. 274: 21645-50. PMID: 10419473
2. Vitamin D3 up-regulated protein 1 mediates oxidative stress via suppressing the thioredoxin function.  |  Junn, E., et al. 2000. J Immunol. 164: 6287-95. PMID: 10843682
3. Cloning, genetic characterization, and chromosomal mapping of the mouse VDUP1 gene.  |  Ludwig, DL., et al. 2001. Gene. 269: 103-12. PMID: 11376942
4. Vitamin D(3) up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species.  |  Song, H., et al. 2003. Immunol Lett. 86: 235-47. PMID: 12706526
5. VDUP1 upregulated by TGF-beta1 and 1,25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression.  |  Han, SH., et al. 2003. Oncogene. 22: 4035-46. PMID: 12821938
6. The expression of vitamin D-upregulated protein 1 in skin and its interaction with sciellin in cultured keratinocytes.  |  Champliaud, MF., et al. 2003. J Invest Dermatol. 121: 781-5. PMID: 14632196
7. Heat shock factor regulates VDUP1 gene expression.  |  Kim, KY., et al. 2004. Biochem Biophys Res Commun. 315: 369-75. PMID: 14766217
8. Rat endometrial Vdup1 expression: changes related to sensitization for the decidual cell reaction and hormonal control.  |  Simmons, DG. and Kennedy, TG. 2004. Reproduction. 127: 475-82. PMID: 15047938
9. VDUP1 is required for the development of natural killer cells.  |  Lee, KN., et al. 2005. Immunity. 22: 195-208. PMID: 15723808
10. Diverse functions of VDUP1 in cell proliferation, differentiation, and diseases.  |  Kim, SY., et al. 2007. Cell Mol Immunol. 4: 345-51. PMID: 17976314
11. VDUP1 Deficiency Promotes the Severity of DSS-Induced Colitis in Mice by Inducing Macrophage Infiltration.  |  Park, KH., et al. 2023. Int J Mol Sci. 24: PMID: 37686390
12. Isolation and characterization of a novel cDNA from HL-60 cells treated with 1,25-dihydroxyvitamin D-3.  |  Chen, KS. and DeLuca, HF. 1994. Biochim Biophys Acta. 1219: 26-32. PMID: 8086474

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VDUP1 Antibody (B-2) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-VDUP1 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VDUP1 Antibody (B-2) Specifications

Alternate Product Name: VDUP1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-115

Epitope Target: VDUP1

Epitope Species: human

Product Reactivity/Detection: VDUP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for VDUP1 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred VDUP1 antibody is VDUP1 Antibody (H-12): sc-271238.