VDR Antibody (D-6) recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and IHC(P)

VDR Antibody (D-6): sc-13133

VDR Antibody (D-6) is rated 4.7 out of 5 by 27.
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Datasheets

    • Anti-VDR Antibody (D-6) is a mouse monoclonal IgG2a (kappa light chain) VDR antibody provided at 200 µg/ml
    • raised against amino acids 344-424 of VDR of human origin
    • Anti-VDR Antibody (D-6) is recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-VDR Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-13133 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VDR (D-6): sc-13133.
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Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Asked by: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Answered by: Tech Support Europe
Date published: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Asked by: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Answered by: Tech Service
Date published: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Asked by: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Asked by: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Answered by: Technical Support Europe
Date published: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Asked by: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Answered by: Technical Support Europe
Date published: 2018-09-01

What is VDR D-6 best diluted in?

Asked by: Daraaaa
Thank you for your question. What it is best diluted in depends on the application you plan on using it for. For Western blot, we would recommend diluting it in 5% milk with TBS-T. For IF/IHC, we would recommend diluting it in blocking serum. You can find more information in the protocol you plan on following: https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Service
Date published: 2017-05-25

Hello, Does this ab work fine when it is conjugated to a regular anti-mouse IgG? Thanks!

Asked by: MMac
Thank you for your question. Yes, this antibody can be used with a conventional anti-mouse IgG secondary or our Mouse IgG Binding Protein, both are appropriate.
Answered by: Technical Service
Date published: 2017-04-24

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-13133 is available directly conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2017-08-28
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Rated 5 out of 5 by from very good antibody This antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date published: 2019-01-02
Rated 5 out of 5 by from Best Antibody We have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Date published: 2018-08-24
Rated 5 out of 5 by from EXCELLENT SENSITIVITY TO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Date published: 2018-04-12
Rated 5 out of 5 by from Works well for immunofluorescence and wester blot This antibody works very well at 1:1000 dilution for western blot and at 1:200 dilution for immunofluorescence on mouse calvariae.
Date published: 2018-03-16
Rated 4 out of 5 by from Arterial immunohistochemistry VDR antibody is a nuclear antibody, but the cytoplasm is also stained. Look at the pictures provided by manual also have cytoplasmic staining. Do you know how to explain properly?
Date published: 2017-10-16
Rated 5 out of 5 by from Good IHC result We usually used this antibody for immunohistochemistry on PFA-fixed VDR of mouse brain,we got the specific staining at dilution 1:150,the result very happy to us.
Date published: 2017-08-12
Rated 5 out of 5 by from Hyperglycemia Enhances Kidney Cell Injury in HIVAN VDR antibody has been succesfully used in our research laboratory . Our work essentially relied on western blotting of selective proteins like VDR.
Date published: 2017-08-07
Rated 5 out of 5 by from Great IP stains Also can be used in IP and IHC, the antibody is good quality.
Date published: 2017-04-17
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