VapH Antibody (F-1): sc-390416
- VapH Antibody (F-1) is a mouse monoclonal IgG1 κ VapH antibody provided at 200 µg/ml
- raised against amino acids 1-187 representing full length virulence associated protein virulence associated protein VapH of Rhodococcus equi origin
- VapH Antibody (F-1) is recommended for detection of virulence associated protein VapH of R. equi subsp. equi origin by WB, IP, IF and ELISA
- Anti-VapH Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VapH Antibody (F-1) for WB applications. These reagents are now offered in bundles with VapH Antibody (F-1) (see ordering information below).
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VapH Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects VapH protein of R. equi subsp. equi origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VapH (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VapH protein is one of seven virulence-associated proteins produced by Rhodococcus equi, a gram-positive bacterium known to cause severe pyogranulomatous pneumonia in foals and pose significant health risks to immunocompromised humans. Understanding VapH structure is crucial, as VapH plays a vital role in the bacterium′s ability to evade host immune response. VapH contains a unique domain that facilitates interaction with host cells, allowing R. equi to survive and replicate within macrophages. This structural feature is essential for R. equi pathogenicity, making VapH a key target for therapeutic interventions and vaccine development. Infected foals typically mount an immune response against R. equi, with antibodies primarily targeting VapA, while responses to VapD, VapF, VapG, and VapH are observed at decreasing levels. VapH (F-1) monoclonal antibody′s ability to accurately detect VapH is invaluable for research focused on understanding R. equi infections and developing effective treatments.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VapH Antibody (F-1) References:
- DNA sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103. | Takai, S., et al. 2000. Infect Immun. 68: 6840-7. PMID: 11083803
- Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins. | Hooper-McGrevy, KE., et al. 2003. Clin Diagn Lab Immunol. 10: 345-51. PMID: 12738629
- Deletion of vapA encoding Virulence Associated Protein A attenuates the intracellular actinomycete Rhodococcus equi. | Jain, S., et al. 2003. Mol Microbiol. 50: 115-28. PMID: 14507368
- Rhodococcus equi secreted antigens are immunogenic and stimulate a type 1 recall response in the lungs of horses immune to R. equi infection. | Kohler, AK., et al. 2003. Infect Immun. 71: 6329-37. PMID: 14573652
- The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701. | Russell, DA., et al. 2004. J Bacteriol. 186: 5576-84. PMID: 15317761
- VapI, a new member of the Rhodococcus equi Vap family. | Polidori, M. and Haas, A. 2006. Antonie Van Leeuwenhoek. 90: 299-304. PMID: 16871422
- Molecular characterization of Rhodococcus equi from horse-breeding farms by means of multiplex PCR for the vap gene family. | Monego, F., et al. 2009. Curr Microbiol. 58: 399-403. PMID: 19205798
- Development of a live, attenuated, potential vaccine strain of R. equi expressing vapA and the virR operon, and virulence assessment in the mouse. | Whitehead, AE., et al. 2012. Vet Immunol Immunopathol. 145: 479-84. PMID: 22088674
- Development of ELISA test for determination of the level of antibodies against Rhodococcus equi in equine serum and colostrum. | Witkowski, L., et al. 2012. Vet Immunol Immunopathol. 149: 280-5. PMID: 22883938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VapH Antibody (F-1) | sc-390416 | 200 µg/ml | $322.00 | |||
VapH Antibody (F-1): m-IgG Fc BP-HRP Bundle | sc-530151 | 200 µg Ab; 10 µg BP | $361.00 | |||
VapH Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-523527 | 200 µg Ab, 40 µg BP | $361.00 | |||
VapH Antibody (F-1): m-IgG1 BP-HRP Bundle | sc-543894 | 200 µg Ab; 20 µg BP | $361.00 | |||
VapH Antibody (F-1) AC | sc-390416 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VapH Antibody (F-1) HRP | sc-390416 HRP | 200 µg/ml | $322.00 | |||
VapH Antibody (F-1) FITC | sc-390416 FITC | 200 µg/ml | $336.00 | |||
VapH Antibody (F-1) PE | sc-390416 PE | 200 µg/ml | $349.00 | |||
VapH Antibody (F-1) Alexa Fluor® 488 | sc-390416 AF488 | 200 µg/ml | $364.00 | |||
VapH Antibody (F-1) Alexa Fluor® 546 | sc-390416 AF546 | 200 µg/ml | $364.00 | |||
VapH Antibody (F-1) Alexa Fluor® 594 | sc-390416 AF594 | 200 µg/ml | $364.00 | |||
VapH Antibody (F-1) Alexa Fluor® 647 | sc-390416 AF647 | 200 µg/ml | $364.00 | |||
VapH Antibody (F-1) Alexa Fluor® 680 | sc-390416 AF680 | 200 µg/ml | $364.00 | |||
VapH Antibody (F-1) Alexa Fluor® 790 | sc-390416 AF790 | 200 µg/ml | $364.00 |