VAP-A Antibody (4C12): sc-293278

VAP-A Antibody (4C12) is a mouse monoclonal IgG2a kappa light chain antibody that detects VAP-A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-VAP-A antibody (4C12) is available as the non-conjugated format. VAMP-associated protein A (VAP-A) is a crucial SNARE regulator that plays a significant role in membrane fusion and intracellular protein transport, particularly during late embryogenesis and early neonatal development in the intestine. VAP-A protein is predominantly located in the endoplasmic reticulum and Golgi apparatus, where VAP-A facilitates the trafficking of vesicles, ensuring proper cellular function and communication. VAP-A protein interacts with a variety of SNAREs and fusion-related proteins, including Syntaxin 1A, rBet1, rSec22, αSNAP, and NSF, indicating VAP-A′s essential role in SNARE-mediated vesicle traffic. VAP-A′s ability to form stable complexes with proteins such as p48 further underscores VAP-A′s importance in modulating cellular processes, including the differentiation of intestinal smooth muscle cells. Using VAP-A monoclonal antibody (4C12) in research can provide valuable insights into the mechanisms of vesicle transport and membrane dynamics in various biological contexts.

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VAP-A Antibody (4C12) References:

1. The chest radiograph in critically ill surgical patients is inaccurate in predicting ventilator-associated pneumonia.  |  Butler, KL., et al. 1999. Am Surg. 65: 805-9; discussion 809-10. PMID: 10484081
2. VAP-A binds promiscuously to both v- and tSNAREs.  |  Weir, ML., et al. 2001. Biochem Biophys Res Commun. 286: 616-21. PMID: 11511104
3. Vesicle-associated membrane protein-associated protein-A (VAP-A) interacts with the oxysterol-binding protein to modify export from the endoplasmic reticulum.  |  Wyles, JP., et al. 2002. J Biol Chem. 277: 29908-18. PMID: 12023275
4. Vesicle-associated protein-A is differentially expressed during intestinal smooth muscle cell differentiation.  |  Gabetta, V., et al. 2003. Dev Dyn. 228: 11-20. PMID: 12950075
5. Norwalk virus nonstructural protein p48 forms a complex with the SNARE regulator VAP-A and prevents cell surface expression of vesicular stomatitis virus G protein.  |  Ettayebi, K. and Hardy, ME. 2003. J Virol. 77: 11790-7. PMID: 14557663
6. Effects of iron modulation on growth and viability of Rhodococcus equi and expression of virulence-associated protein A.  |  Jordan, MC., et al. 2003. Am J Vet Res. 64: 1337-46. PMID: 14620767
7. VAMP-associated protein-A regulates partitioning of oxysterol-binding protein-related protein-9 between the endoplasmic reticulum and Golgi apparatus.  |  Wyles, JP. and Ridgway, ND. 2004. Exp Cell Res. 297: 533-47. PMID: 15212954
8. VAP, a Versatile Access Point for the Endoplasmic Reticulum: Review and analysis of FFAT-like motifs in the VAPome.  |  Murphy, SE. and Levine, TP. 2016. Biochim Biophys Acta. 1861: 952-961. PMID: 26898182
9. Nanoscale architecture of a VAP-A-OSBP tethering complex at membrane contact sites.  |  de la Mora, E., et al. 2021. Nat Commun. 12: 3459. PMID: 34103503
10. VAP-A and its binding partner CERT drive biogenesis of RNA-containing extracellular vesicles at ER membrane contact sites.  |  Barman, B., et al. 2022. Dev Cell. 57: 974-994.e8. PMID: 35421371
11. VAP-A intrinsically disordered regions enable versatile tethering at membrane contact sites.  |  Subra, M., et al. 2023. Dev Cell. 58: 121-138.e9. PMID: 36693319
12. Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins.  |  Nishimura, Y., et al. 1999. Biochem Biophys Res Commun. 254: 21-6. PMID: 9920726

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VAP-A Antibody (4C12) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-VAP-A Antibody (4C12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VAP-A Antibody (4C12) Specifications

Alternate Product Name: VAP-A

Product Clone Name: 4C12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-242

Epitope Target: VAP-A

Epitope Species: human

Product Reactivity/Detection: VAP-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for VAP-A Antibody (4C12): N/A