vanin-1 CRISPR/Cas9 KO Plasmid (h): sc-406512
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- vanin-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the vanin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: vanin-1 Antibody (3-RE8): sc-135599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
vanin-1 CRISPR/Cas9 KO Plasmid (h) | sc-406512 | 20 µg | $397.00 |
Overview
VNN1 encodes vanin-1, a GPI-anchored ectoenzyme with pantetheinase activity that hydrolyzes pantetheine to generate cysteamine and pantothenate, linking coenzyme A metabolism to redox control. Through modulation of cysteamine/cystamine balance and downstream effects on glutathione homeostasis, vanin-1 influences cellular responses to oxidative stress, inflammation, and tissue injury. VNN1 expression is prominent in epithelial and immune-relevant contexts, where it can shape leukocyte recruitment and barrier-associated inflammatory signaling. Dysregulated VNN1/vanin-1 activity has been associated with inflammatory and metabolic disease states, making it a useful node for studying stress-responsive pathways.
vanin-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VNN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VNN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VNN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish vanin-1 protein expression.
This CRISPR knockout system enables efficient generation of VNN1-deficient cell models for investigation of vanin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VNN1 exon(s) critical for vanin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VNN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by vanin-1 CRISPR/Cas9 KO Plasmid (h) and vanin-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VNN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by vanin-1 HDR Plasmid (h) and vanin-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VNN1 homology arms to support homology-directed repair at defined VNN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.