VAMP-3 Antibody (E-10): sc-514843

VAMP-3 Antibody (E-10) is a mouse monoclonal IgM antibody that detects VAMP-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-VAMP-3 antibody (E-10) is available in a non-conjugated form, allowing for versatile experimental use. VAMP-3, also known as cellubrevin, is a member of the vesicle-associated membrane protein (VAMP) family, which plays a crucial role in the regulation of vesicle trafficking and exocytosis. VAMP-3 is primarily located in the membranes of secretory vesicles and is essential for the fusion of these vesicles with target membranes, a process vital for neurotransmitter release and hormone secretion. VAMP-3 functions particularly in pancreatic beta-cells, where VAMP-3 is involved in the calcium-mediated release of insulin, thereby influencing glucose homeostasis. Additionally, VAMP-3 interacts with synaptosomal-associated proteins (SNAPs) and syntaxin, forming a complex that is critical for the docking and fusion of vesicles, highlighting VAMP-3′s significance in cellular communication and metabolic regulation. VAMP-3′s ability to facilitate the translocation of GLUT4 to the cell surface in adipocytes further underscores VAMP-3′s role in insulin signaling and glucose uptake, making VAMP-3 a key player in metabolic processes.

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VAMP-3 Antibody (E-10) References:

1. Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion.  |  Luftman, K., et al. 2009. Biochem Biophys Res Commun. 380: 65-70. PMID: 19159614
2. SNAP-23 and VAMP-3 contribute to the release of IL-6 and TNFα from a human synovial sarcoma cell line.  |  Boddul, SV., et al. 2014. FEBS J. 281: 750-65. PMID: 24373201
3. Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking.  |  Li, D., et al. 2015. J Physiol. 593: 2807-32. PMID: 25864578
4. VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.  |  Zhu, JJ., et al. 2020. Front Cell Dev Biol. 8: 576826. PMID: 33224946
5. VAMP-2 and cellubrevin are expressed in pancreatic beta-cells and are essential for Ca(2+)-but not for GTP gamma S-induced insulin secretion.  |  Regazzi, R., et al. 1995. EMBO J. 14: 2723-30. PMID: 7796801
6. Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.  |  Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
7. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site.  |  McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
8. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.  |  Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
9. A role for synaptotagmin (p65) in regulated exocytosis.  |  Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
10. Cleavage of vesicle-associated membrane protein (VAMP)-2 and cellubrevin on GLUT4-containing vesicles inhibits the translocation of GLUT4 in 3T3-L1 adipocytes.  |  Tamori, Y., et al. 1996. Biochem Biophys Res Commun. 220: 740-5. PMID: 8607835
11. Syntaxin 4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes.  |  Olson, AL., et al. 1997. Mol Cell Biol. 17: 2425-35. PMID: 9111311
12. Structural organization of the synaptic exocytosis core complex.  |  Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VAMP-3 Antibody (E-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-VAMP-3 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VAMP-3 Antibody (E-10) Specifications

Alternate Product Name: VAMP-3

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2-16

Epitope Target: VAMP-3

Epitope Species: human

Product Reactivity/Detection: VAMP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for VAMP-3 Antibody (E-10): P: Blocking Peptide

The preferred VAMP-1/2/3 antibody is VAMP-1/2/3 Antibody (F-11): sc-133129.