VAMP-1/2/3 Antibody (F-11): sc-133129

VAMP-1/2/3 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects VAMP-1/2/3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). VAMP-1/2/3 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VAMP proteins, also known as synaptobrevins, play a crucial role in exocytosis, which is essential for neurotransmitter release and other cellular secretion mechanisms. VAMP proteins are primarily located on synaptic vesicle membranes and are vital for vesicle fusion with presynaptic membrane, facilitating neurotransmitter release into synaptic cleft. VAMP interaction with syntaxins and SNAPs is critical for SNARE complex formation, which drives membrane fusion. Disruption of this process can lead to various neurological disorders, highlighting VAMP protein importance in maintaining proper synaptic function. Using anti-VAMP-1/2/3 antibody (F-11) in research can provide valuable insights into exocytosis mechanisms and implications in cellular communication and pathology.

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VAMP-1/2/3 Antibody (F-11) References:

1. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion.  |  Polgár, J., et al. 2002. Blood. 100: 1081-3. PMID: 12130530
2. The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their correct subcellular targeting: the N-terminal extenSion of VAMP4 contains a dominant autonomous targeting signal for the trans-Golgi network.  |  Zeng, Q., et al. 2003. J Biol Chem. 278: 23046-54. PMID: 12682051
3. The variable C-terminus of cysteine string proteins modulates exocytosis and protein-protein interactions.  |  Boal, F., et al. 2004. Biochemistry. 43: 16212-23. PMID: 15610015
4. VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.  |  Tajika, Y., et al. 2007. Cell Tissue Res. 328: 573-81. PMID: 17468895
5. VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes.  |  Ferlito, M., et al. 2010. J Mol Cell Cardiol. 49: 791-800. PMID: 20801128
6. Specificity of botulinum protease for human VAMP family proteins.  |  Yamamoto, H., et al. 2012. Microbiol Immunol. 56: 245-53. PMID: 22289120
7. Differential regulation of the serotonin transporter by vesicle-associated membrane protein 2 in cells of neuronal versus non-neuronal origin.  |  Müller, HK., et al. 2014. PLoS One. 9: e97540. PMID: 24878716
8. Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons.  |  Hoogstraaten, RI., et al. 2020. Sci Rep. 10: 10913. PMID: 32616842
9. VAMPs sensitive to tetanus toxin are required for cortical granule exocytosis in mouse oocytes.  |  de Paola, M., et al. 2021. Exp Cell Res. 405: 112629. PMID: 34023392
10. The intramembrane proteases SPPL2a and SPPL2b regulate the homeostasis of selected SNARE proteins.  |  Ballin, M., et al. 2023. FEBS J. 290: 2320-2337. PMID: 36047592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VAMP-1/2/3 Antibody (F-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-VAMP-1/2/3 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VAMP-1/2/3 Antibody (F-11) Specifications

Alternate Product Name: Synaptobrevin

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-118

Epitope Target: VAMP-1

Epitope Species: human

Product Reactivity/Detection: VAMP-1, VAMP-2 and VAMP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for VAMP-1/2/3 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin