VAMP-2 Antibody (3E5) recommended for detection of VAMP-2 of mouse, rat and human origin by WB and IP

VAMP-2 Antibody (3E5): sc-69706

VAMP-2 Antibody (3E5) is rated 3.8 out of 5 by 4.
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Datasheets

    • VAMP-2 Antibody (3E5) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against a recombinant protein corresponding to amino acids 1-89 of VAMP-2 of human origin by WB and IP
    • recommended for detection of VAMP-2 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VAMP-2 (3E5): sc-69706.

I am using VAMP-2 (3E5): sc-69706 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked well by WB Worked well by Westren blot. SCBT Publication Review
Date published: 2019-11-14
Rated 5 out of 5 by from Great for Western blot VAMP-2 Antibody (3E5) produced strong and positive results by Western blot
Date published: 2017-01-27
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-07-02
Rated 3 out of 5 by from Publishable data in WB using rat SNs Publishable data in WB using rat SNs -SCBT Publication Review
Date published: 2014-06-14
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