V5-Probe Antibody (C-9): sc-271944

V5-Probe Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize the V5 epitope, also known as the Pk or SV5 tag, which spans amino acids 87-111 within the C-terminal region of the P and V proteins from Simian Virus 5 (SV5). The V5 epitope is a linear peptide commonly fused to recombinant proteins to facilitate their detection and purification in various experimental settings. The structured nature of the V5 epitope allows incorporation into fusion proteins without disrupting native conformation or function of target proteins, making V5-Probe (C-9) antibody an invaluable tool for protein studies. Understanding V5-tagged protein structure is essential for accurately assessing protein localization, interactions, and functionality within different cellular environments. V5-Probe monoclonal antibody (C-9) is compatible with multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), providing researchers with versatile options for experimental needs. Anti-V5-Probe antibody (C-9) supports expression of eukaryotic genes in bacterial, insect, and mammalian systems, enabling study of a wide range of recombinant proteins, including transmembrane and secreted proteins. Available in both non-conjugated and various conjugated forms such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, V5-Probe (C-9) antibody accommodates diverse detection methods and experimental requirements. Recognition of V5 epitope facilitates visualization and analysis of tagged proteins, thereby aiding advancement of research in molecular biology, cell biology, and biotechnology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V5-Probe Antibody (C-9) has 30 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V5-Probe Antibody (C-9) Specifications

Alternate Product Name: V5 tag

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 87-111

Epitope Target: the P and V proteins

Epitope Species: Simian Virus 5

Product Reactivity/Detection: V5 fusion proteins

Species Reactivity/Detection: n/a

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for V5-Probe Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred V5-Probe antibody is V5-Probe Antibody (E10): sc-81594.