Date published: 2026-3-16

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V-ATPase H Antibody (E-10): sc-271186

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Datasheets
  • V-ATPase H Antibody (E-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 10-41 at the N-terminus of V-ATPase H of human origin
  • recommended for detection of V-ATPase H of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
  • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase H Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase H Antibody (E-10) (see ordering information below).
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    V-ATPase H Antibody (E-10) is a mouse monoclonal IgG2a antibody that detects V-ATPase H in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase H, also known as the H subunit of the vacuolar-type H+-ATPase, is a crucial regulatory component of this multisubunit enzyme, which is essential for the acidification of eukaryotic intracellular organelles. V-ATPase H is located in the membrane of these organelles, where V-ATPase H plays a vital role in maintaining pH homeostasis and facilitating various cellular processes, such as nutrient uptake and waste removal. The H subunit is composed of two polypeptides derived from the same gene, and proper functioning is critical for the coupling of ATP hydrolysis to proton transport, thereby ensuring efficient energy conversion within the cell. Anti-V-ATPase H antibody (E-10) enables researchers to gain insights into the dynamics of proton transport and the overall functionality of the V-ATPase complex, which is pivotal for cellular health and metabolism.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    V-ATPase H Antibody (E-10)

    sc-271186
    200 µg/ml
    $322.00

    V-ATPase H Antibody (E-10): m-IgGκ BP-HRP Bundle

    sc-534888
    200 µg Ab; 40 µg BP
    $361.00

    V-ATPase H Antibody (E-10): m-IgG2a BP-HRP Bundle

    sc-546199
    200 µg Ab; 10 µg BP
    $361.00

    V-ATPase H (E-10) Neutralizing Peptide

    sc-271186 P
    100 µg/0.5 ml
    $69.00

    What application is the blocking peptide sc-271186 P appropriate for?

    Asked by: jenniferc
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-24
    • y_2026, m_3, d_10, h_8CST
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    Rated 5 out of 5 by from Positive detection of endogenously expressed VPositive detection of endogenously expressed V-ATPase H protein in DU 145 cells by WB. -SCBT QC
    Date published: 2015-03-07
    Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
    Date published: 2015-03-01
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    V-ATPase H Antibody (E-10) is rated 5.0 out of 5 by 2.
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