V-ATPase D1 Antibody (D-4): sc-393322
- V-ATPase D1 Antibody (D-4) is a mouse monoclonal IgG1 κ V-ATPase D1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 66-105 mapping within an internal region of V-ATPase D1 of human origin
- V-ATPase D1 Antibody (D-4) is recommended for detection of V-ATPase D1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-V-ATPase D1 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for V-ATPase D1 Antibody (D-4) for WB applications. These reagents are now offered in bundles with V-ATPase D1 Antibody (D-4) (see ordering information below).
QUICK LINKS
SEE ALSO...
V-ATPase D1 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase D1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase D1 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. V-ATPase D1, also known as ATP6V0D1, plays a crucial role in acidifying eukaryotic intracellular organelles, which is essential for numerous cellular processes, including protein degradation, nutrient storage, and pH regulation within organelles. ATP6V0D1 is located in lysosome and endosome membranes, where ATP6V0D1 facilitates proton transport across membranes, creating an acidic environment necessary for enzyme activation and proper cellular compartment functioning. V-ATPase complex consists of two main domains: peripheral V1 domain responsible for ATP hydrolysis, and integral V0 domain involved in proton translocation. V0 domain′s D subunit, V-ATPase D1, interacts with other subunits to form a functional enzyme vital for maintaining cellular homeostasis and facilitating vacuolar transport.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
V-ATPase D1 Antibody (D-4) References:
- Structure and properties of the vacuolar (H+)-ATPases. | Forgac, M. 1999. J Biol Chem. 274: 12951-4. PMID: 10224039
- Structure of the VPATPD gene encoding subunit D of the human vacuolar proton ATPase. | Agarwal, AK. and White, PC. 2000. Biochem Biophys Res Commun. 279: 543-7. PMID: 11118322
- The vacuolar (H+)-ATPases--nature's most versatile proton pumps. | Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
- Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis. | Smith, AN., et al. 2002. Gene. 297: 169-77. PMID: 12384298
- Proton translocation driven by ATP hydrolysis in V-ATPases. | Kawasaki-Nishi, S., et al. 2003. FEBS Lett. 545: 76-85. PMID: 12788495
- Neurotransmitter release: the dark side of the vacuolar-H+ATPase. | Morel, N. 2003. Biol Cell. 95: 453-7. PMID: 14597263
- Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis. | Pietrement, C., et al. 2006. Biol Reprod. 74: 185-94. PMID: 16192400
- Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase. | van Hille, B., et al. 1993. Biochem Biophys Res Commun. 197: 15-21. PMID: 8250920
- The vacuolar H+-ATPase: a universal proton pump of eukaryotes. | Finbow, ME. and Harrison, MA. 1997. Biochem J. 324 (Pt 3): 697-712. PMID: 9210392
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
V-ATPase D1 Antibody (D-4) | sc-393322 | 200 µg/ml | $322.00 | |||
V-ATPase D1 Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-527680 | 200 µg Ab; 10 µg BP | $361.00 | |||
V-ATPase D1 Antibody (D-4): m-IgG1 BP-HRP Bundle | sc-533053 | 200 µg Ab; 20 µg BP | $361.00 | |||
V-ATPase D1 Antibody (D-4) AC | sc-393322 AC | 500 µg/ml, 25% agarose | $424.00 | |||
V-ATPase D1 Antibody (D-4) HRP | sc-393322 HRP | 200 µg/ml | $322.00 | |||
V-ATPase D1 Antibody (D-4) FITC | sc-393322 FITC | 200 µg/ml | $336.00 | |||
V-ATPase D1 Antibody (D-4) PE | sc-393322 PE | 200 µg/ml | $349.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 488 | sc-393322 AF488 | 200 µg/ml | $364.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 546 | sc-393322 AF546 | 200 µg/ml | $364.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 594 | sc-393322 AF594 | 200 µg/ml | $364.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 647 | sc-393322 AF647 | 200 µg/ml | $364.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 680 | sc-393322 AF680 | 200 µg/ml | $364.00 | |||
V-ATPase D1 Antibody (D-4) Alexa Fluor® 790 | sc-393322 AF790 | 200 µg/ml | $364.00 |