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V-ATPase D Antibody (D-4) - Western Blotting - Image 374700
V-ATPase D Antibody (D-4): sc-166218. Western blot analysis of V-ATPase D expression in SH-SY5Y (A) and C6 (B) whole cell lysates and mouse brain tissue extract (C).
V-ATPase D Antibody (D-4): sc-166218. Western blot analysis of V-ATPase D expression in SH-SY5Y (A) and C6 (B) whole cell lysates and mouse brain tissue extract (C).

V-ATPase D Antibody (D-4): sc-166218

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Datasheets
  • V-ATPase D Antibody (D-4) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
  • raised against amino acids 1-243 representing full length V-ATPase D of human origin
  • recommended for detection of V-ATPase D of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG1 BP-HRP is the preferred secondary detection reagent for V-ATPase D Antibody (D-4) for WB applications. This reagent is now offered in a bundle with V-ATPase D Antibody (D-4) (see ordering information below).
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Additional V-ATPase Antibodies including V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1, V-ATPase G2 and V-ATPase H

V-ATPase D Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects V-ATPase D in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). V-ATPase D is a crucial component of the vacuolar-type H+-ATPase complex, which plays a vital role in the acidification of intracellular organelles, a process essential for various cellular functions including protein sorting, nutrient storage, and pH regulation within organelles. The V-ATPase complex is composed of two main domains: the peripheral V1 domain, responsible for ATP hydrolysis, and the integral V0 domain, which facilitates proton translocation across membranes. The V-ATPase D subunit, part of the V0 domain, is particularly important for the assembly and stability of the entire complex, ensuring efficient proton pumping and maintaining the electrochemical gradient necessary for ATP synthesis. Proper functioning of V-ATPase D is critical for cellular homeostasis, and dysregulation has been implicated in various diseases, including cancer and neurodegenerative disorders. Anti-V-ATPase D antibody (D-4) provides a reliable tool for researchers studying the dynamics of this essential protein and its role in cellular physiology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

V-ATPase D Antibody (D-4) References:

  1. Expression and function of the mouse V-ATPase d subunit isoforms.  |  Nishi, T., et al. 2003. J Biol Chem. 278: 46396-402. PMID: 12963731
  2. The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion.  |  Xia, Y., et al. 2019. Autophagy. 15: 960-975. PMID: 30681394
  3. Phosphorylated tau interactome in the human Alzheimer's disease brain.  |  Drummond, E., et al. 2020. Brain. 143: 2803-2817. PMID: 32812023
  4. Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase.  |  Zhou, X., et al. 2022. Acta Pharm Sin B. 12: 3843-3860. PMID: 36213534
  5. ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.  |  Chen, F., et al. 2023. Cell Rep. 42: 111911. PMID: 36640329
  6. A Novel Somatic Mutation Implicates ATP6V0D1 in Proinsulin Processing.  |  Avari, P., et al. 2023. J Endocr Soc. 7: bvac196. PMID: 36694809
  7. Mechanisms of alkaliptosis.  |  Chen, F., et al. 2023. Front Cell Dev Biol. 11: 1213995. PMID: 37601110
  8. Myocardin reverses insulin resistance and ameliorates cardiomyopathy by increasing IRS-1 expression in a murine model of lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase V0d1 subunit.  |  Yuan, W., et al. 2024. Theranostics. 14: 2246-2264. PMID: 38505620
  9. Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition.  |  Chen, F., et al. 2024. Mol Carcinog. 63: 1515-1527. PMID: 38751020
  10. Physical Exercise Decreases Complement-Mediated Synaptic Loss and Protects Against Cognitive Impairment by Inhibiting Microglial Tmem9-ATP6V0D1 in Alzheimer's Disease.  |  Li, S., et al. 2025. Aging Cell. e14496. PMID: 39871402

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

V-ATPase D Antibody (D-4)

sc-166218
200 µg/ml
$322.00

V-ATPase D Antibody (D-4): m-IgG1 BP-HRP Bundle

sc-542659
200 µg Ab; 20 µg BP
$361.00

What is the recommended fixation method for immunofluorescence staining with V-ATPase D (D-4): sc-166218 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Positive detection of endogenous proteinPositive detection of endogenous protein expression levels of the V-ATPase D protein in mouse and rat brain tissue extracts and SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Publishable results obtained by WB usingPublishable results obtained by WB using human Huh7 cells. -SCBT Publication Review
Date published: 2015-01-29
Rated 5 out of 5 by from Product scProduct sc-166218 has been reported to be suitable for use in WB using mouse samples. -SCBT Publication Review
Date published: 2015-01-16
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V-ATPase D Antibody (D-4) is rated 4.8 out of 5 by 4.
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