V-ATPase α1 Antibody (4F5): sc-293336

V-ATPase α1 Antibody (4F5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the V-ATPase alpha 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase alpha 1 antibody (4F5) is available as the non-conjugated format. The vacuolar-type H+-ATPase (V-ATPase) plays a crucial role in maintaining cellular homeostasis by acidifying various intracellular organelles, which is essential for processes such as protein degradation, nutrient storage, and ion transport. The V-ATPase operates through a complex structure consisting of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation. The V1 domain is composed of nine subunits (A–H), while the V0 domain consists of five subunits (a, d, c, c′, and c"). V-ATPase α1, also known as HO68, VA68, VPP2, Vma1, or ATP6V1A1, serves as the A subunit of the V1 domain and is a 617 amino acid protein that is ubiquitously expressed across various tissues. V-ATPase α1 proper functioning is vital for the enzyme′s rotary mechanism, which couples ATP hydrolysis to proton translocation, thereby influencing numerous physiological processes.

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V-ATPase α1 Antibody (4F5) References:

1. The vacuolar (H+)-ATPases--nature's most versatile proton pumps.  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
2. Proton translocation driven by ATP hydrolysis in V-ATPases.  |  Kawasaki-Nishi, S., et al. 2003. FEBS Lett. 545: 76-85. PMID: 12788495
3. Neurotransmitter release: the dark side of the vacuolar-H+ATPase.  |  Morel, N. 2003. Biol Cell. 95: 453-7. PMID: 14597263
4. Phosphatidylinositol 3-kinase-mediated effects of glucose on vacuolar H+-ATPase assembly, translocation, and acidification of intracellular compartments in renal epithelial cells.  |  Sautin, YY., et al. 2005. Mol Cell Biol. 25: 575-89. PMID: 15632060
5. Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis.  |  Pietrement, C., et al. 2006. Biol Reprod. 74: 185-94. PMID: 16192400
6. Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio).  |  Horng, JL., et al. 2007. Am J Physiol Regul Integr Comp Physiol. 292: R2068-76. PMID: 17272665
7. Identification of two subunit A isoforms of the vacuolar H(+)-ATPase in human osteoclastoma.  |  van Hille, B., et al. 1993. J Biol Chem. 268: 7075-80. PMID: 8463241

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase α1 Antibody (4F5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase α1 Antibody (4F5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase α1 Antibody (4F5) Specifications

Alternate Product Name: V-ATPase α1

Product Clone Name: 4F5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 508-617

Epitope Target: V-ATPase α1

Epitope Species: human

Product Reactivity/Detection: V-ATPase α1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for V-ATPase α1 Antibody (4F5): N/A