utrophin Antibody (8A4): sc-33700

utrophin Antibody (8A4) is a mouse monoclonal IgG1 kappa light chain antibody that detects utrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-utrophin antibody (8A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Utrophin plays a crucial role in muscle function and cellular integrity, as utrophin is a structural, actin-binding protein that helps anchor the cytoskeleton to the plasma membrane, similar to dystrophin, which is associated with Duchenne and Becker muscular dystrophies. Utrophin′s importance lies in its ability to compensate for the loss of dystrophin in muscle tissues, particularly in muscular dystrophies. Research indicates that upregulating utrophin can mitigate the pathological effects of dystrophin deficiency, providing a potential therapeutic avenue for treating these debilitating conditions. Additionally, utrophin′s interaction with various proteins, including dystrophin-associated glycoproteins, highlights utrophin′s significance in maintaining muscle fiber structural integrity and facilitating communication between the cytoskeleton and extracellular matrix.

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utrophin Antibody (8A4) References:

1. Regulation and functional significance of utrophin expression at the mammalian neuromuscular synapse.  |  Gramolini, AO., et al. 2000. Microsc Res Tech. 49: 90-100. PMID: 10757882
2. Dystrophin as a diagnostic marker in Duchenne and Becker muscular dystrophy. Correlation of immunofluorescence and western blot.  |  Voit, T., et al. 1991. Neuropediatrics. 22: 152-62. PMID: 1944822
3. Dystrophin, the protein product of the Duchenne/Becker muscular dystrophy gene.  |  Monaco, AP. 1989. Trends Biochem Sci. 14: 412-5. PMID: 2683261
4. Utrophin influences mitochondrial pathology and oxidative stress in dystrophic muscle.  |  Kennedy, TL., et al. 2017. Skelet Muscle. 7: 22. PMID: 29065908
5. Utrophin modulator drugs as potential therapies for Duchenne and Becker muscular dystrophies.  |  Soblechero-Martín, P., et al. 2021. Neuropathol Appl Neurobiol. 47: 711-723. PMID: 33999469
6. Discovery of Quinazoline and Quinoline-Based Small Molecules as Utrophin Upregulators via AhR Antagonism for the Treatment of Duchenne Muscular Dystrophy.  |  Ghosh, S., et al. 2024. J Med Chem. 67: 9260-9276. PMID: 38771158
7. Utrophin actin binding domain: analysis of actin binding and cellular targeting.  |  Winder, SJ., et al. 1995. J Cell Sci. 108 (Pt 1): 63-71. PMID: 7738117
8. Dystrophin-associated glycoproteins: their possible roles in the pathogenesis of Duchenne muscular dystrophy.  |  Ervasti, JM. and Campbell, KP. 1993. Mol Cell Biol Hum Dis Ser. 3: 139-66. PMID: 8111538
9. Molecular organization at the glycoprotein-complex-binding site of dystrophin. Three dystrophin-associated proteins bind directly to the carboxy-terminal portion of dystrophin.  |  Suzuki, A., et al. 1994. Eur J Biochem. 220: 283-92. PMID: 8125086
10. A new model for the interaction of dystrophin with F-actin.  |  Rybakova, IN., et al. 1996. J Cell Biol. 135: 661-72. PMID: 8909541
11. Amelioration of the dystrophic phenotype of mdx mice using a truncated utrophin transgene.  |  Tinsley, JM., et al. 1996. Nature. 384: 349-53. PMID: 8934518
12. Muscle and neural isoforms of agrin increase utrophin expression in cultured myotubes via a transcriptional regulatory mechanism.  |  Gramolini, AO., et al. 1998. J Biol Chem. 273: 736-43. PMID: 9422725

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-utrophin Antibody (8A4) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-utrophin Antibody (8A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

utrophin Antibody (8A4) Specifications

Alternate Product Name: utrophin

Product Clone Name: 8A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: utrophin

Epitope Species: human

Product Reactivity/Detection: utrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for utrophin Antibody (8A4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin