USP4 Antibody (H-3): sc-376000

USP4 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-USP4 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP4, also known as ubiquitin-specific-processing protease 4, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating protein degradation and maintaining cellular homeostasis. Specifically, USP4 removes ubiquitin moieties from target proteins, preventing their degradation and allowing for the stabilization of key signaling molecules. This function is particularly important in various cellular processes, including signal transduction, transcriptional regulation, and cell cycle progression, as USP4 helps modulate the activity and availability of proteins critical for these pathways. Additionally, USP4 interacts with the C-terminus of the Adenosine A2A receptor, enhancing cell surface expression and functional activity, which underscores USP4′s significance in receptor signaling and potential implications in therapeutic targets for diseases related to dysregulated signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

USP4 Antibody (H-3) References:

1. The de-ubiquitinating enzyme Unp interacts with the retinoblastoma protein.  |  DeSalle, LM., et al. 2001. Oncogene. 20: 5538-42. PMID: 11571652
2. Nuclear-cytoplasmic shuttling of the oncogenic mouse UNP/USP4 deubiquitylating enzyme.  |  Soboleva, TA., et al. 2005. J Biol Chem. 280: 745-52. PMID: 15494318
3. The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor.  |  Milojevic, T., et al. 2006. Mol Pharmacol. 69: 1083-94. PMID: 16339847
4. Adenosine receptors find a new partner and move out.  |  Toews, ML. 2006. Mol Pharmacol. 69: 1075-8. PMID: 16421289
5. UnpEL/Usp4 is ubiquitinated by Ro52 and deubiquitinated by itself.  |  Wada, K. and Kamitani, T. 2006. Biochem Biophys Res Commun. 342: 253-8. PMID: 16472766
6. A tail of two signals: the C terminus of the A(2A)-adenosine receptor recruits alternative signaling pathways.  |  Gsandtner, I. and Freissmuth, M. 2006. Mol Pharmacol. 70: 447-9. PMID: 16707626
7. The A(2A)-adenosine receptor: a GPCR with unique features?  |  Zezula, J. and Freissmuth, M. 2008. Br J Pharmacol. 153 Suppl 1: S184-90. PMID: 18246094
8. The human UNP locus at 3p21.31 encodes two tissue-selective, cytoplasmic isoforms with deubiquitinating activity that have reduced expression in small cell lung carcinoma cell lines.  |  Frederick, A., et al. 1998. Oncogene. 16: 153-65. PMID: 9464533
9. Deubiquitinating enzymes: a new class of biological regulators.  |  D'Andrea, A. and Pellman, D. 1998. Crit Rev Biochem Mol Biol. 33: 337-52. PMID: 9827704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP4 Antibody (H-3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-USP4 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP4 Antibody (H-3) Specifications

Alternate Product Name: USP4

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 708-779

Epitope Target: USP4

Epitope Species: human

Product Reactivity/Detection: USP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for USP4 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin