UPIIIa Antibody (C-6): sc-166808

UPIIIa Antibody (C-6) is a mouse monoclonal IgG2a antibody that detects UPIIIa of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UPIIIa (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UPIIIa plays a crucial role in forming the asymmetric unit membrane (AUM), which is essential for maintaining urothelium integrity. AUM is characterized by unique plaques covering the apical surface of the bladder′s urothelium, providing structural support and preventing rupture during bladder distention. These plaques are composed of uroplakins, including UPIa, UPIb, UPII, and UPIII, which are highly conserved across species such as humans, mice, rats, rabbits, dogs, pigs, and sheep. The interaction between UPIa and UPIb with UPII and UPIII is vital for normal urothelial plaque formation, and this process is regulated by proteolytic processing of uroplakin proteins. Uroplakins are not only markers of urothelial differentiation but are also expressed in many transitional cell carcinomas of the bladder, making anti-UPIIIa antibody (C-6) valuable for detecting bladder cancer metastasis and monitoring therapeutic responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

UPIIIa Antibody (C-6) References:

1. Detection of circulating uroplakin-positive cells in patients with transitional cell carcinoma of the bladder.  |  Li, SM., et al. 1999. J Urol. 162: 931-5. PMID: 10458411
2. Uroplakin and androgen receptor expression in the human fetal genital tract: insights into the development of the vagina.  |  Shapiro, E., et al. 2000. J Urol. 164: 1048-51. PMID: 10958738
3. Ablation of uroplakin III gene results in small urothelial plaques, urothelial leakage, and vesicoureteral reflux.  |  Hu, P., et al. 2000. J Cell Biol. 151: 961-72. PMID: 11085999
4. Organization of uroplakin subunits: transmembrane topology, pair formation and plaque composition.  |  Liang, FX., et al. 2001. Biochem J. 355: 13-8. PMID: 11256943
5. Assembly of urothelial plaques: tetraspanin function in membrane protein trafficking.  |  Hu, CC., et al. 2005. Mol Biol Cell. 16: 3937-50. PMID: 15958488
6. Urothelial differentiation in vesicoureteric reflux and other urological disorders of childhood: a comparative study.  |  Garthwaite, MA., et al. 2006. Eur Urol. 49: 154-9, 159-60. PMID: 16314030
7. Distinct glycan structures of uroplakins Ia and Ib: structural basis for the selective binding of FimH adhesin to uroplakin Ia.  |  Xie, B., et al. 2006. J Biol Chem. 281: 14644-53. PMID: 16567801
8. Origin of the tetraspanin uroplakins and their co-evolution with associated proteins: implications for uroplakin structure and function.  |  Garcia-España, A., et al. 2006. Mol Phylogenet Evol. 41: 355-67. PMID: 16814572
9. Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence.  |  Liao, Y., et al. 2018. Mol Biol Cell. 29: 3128-3143. PMID: 30303751
10. Precursor sequence, processing, and urothelium-specific expression of a major 15-kDa protein subunit of asymmetric unit membrane.  |  Lin, JH., et al. 1994. J Biol Chem. 269: 1775-84. PMID: 7507484
11. Mammalian uroplakins. A group of highly conserved urothelial differentiation-related membrane proteins.  |  Wu, XR., et al. 1994. J Biol Chem. 269: 13716-24. PMID: 8175808
12. Selective interactions of UPIa and UPIb, two members of the transmembrane 4 superfamily, with distinct single transmembrane-domained proteins in differentiated urothelial cells.  |  Wu, XR., et al. 1995. J Biol Chem. 270: 29752-9. PMID: 8530366

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UPIIIa Antibody (C-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-UPIIIa Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UPIIIa Antibody (C-6) Specifications

Alternate Product Name: UPIIIa

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids: 21-200

Epitope Target: UPIIIa

Epitope Species: human

Product Reactivity/Detection: UPIIIa

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for UPIIIa Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin