UPIb CRISPR/Cas9 KO Plasmid (m): sc-423620
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UPIb CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UPIb genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UPIb Antibody (1E1): sc-517025
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UPIb CRISPR/Cas9 KO Plasmid (m) | sc-423620 | 20 µg | $397.00 |
Overview
Upk1b encodes uroplakin Ib (UPIb), a tetraspanin-like membrane protein that assembles with other uroplakins to form the asymmetric unit membrane plaques of differentiated urothelial umbrella cells. These plaques support apical membrane organization, barrier integrity, and vesicle trafficking processes that regulate bladder surface remodeling during stretch and voiding. UPIb participates in epithelial differentiation programs and membrane microdomain organization, linking cytoskeletal architecture to junctional and surface protein stability. Altered uroplakin expression patterns are widely used as indicators of urothelial state and are relevant to studies of barrier dysfunction, inflammation, and urothelial transformation.
UPIb CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Upk1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Upk1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Upk1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UPIb protein expression.
This CRISPR knockout system enables efficient generation of Upk1b-deficient cell models for investigation of UPIb signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Upk1b exon(s) critical for UPIb function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Upk1b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UPIb CRISPR/Cas9 KO Plasmid (m) and UPIb CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Upk1b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UPIb HDR Plasmid (m) and UPIb HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Upk1b homology arms to support homology-directed repair at defined Upk1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.