Unc18-3 Antibody (H-7): sc-373813

Unc18-3 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Unc18-3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Unc18-3 Antibody (H-7) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Unc18-3, part of the Unc18 family of proteins, plays a crucial role in regulating exocytosis by acting as a chaperone that prevents syntaxin from interacting with cognate SNARE proteins, thereby ensuring proper vesicle fusion and neurotransmitter release. This regulation is vital for various physiological processes, including neurotransmission and hormone secretion. Unc18-3 is predominantly localized in the cytoplasm and interacts with syntaxin proteins, which are essential for the formation of the SNARE complex that drives vesicle fusion. Additionally, Unc18-3 is expressed in various cell types, including mast cells and platelets, highlighting its importance in cellular communication and signaling pathways. The presence of specific phosphorylation sites on Unc18-1, such as Ser 313 and Thr 574, further underscores the complexity of regulation and interaction with syntaxin, making anti-Unc18-3 antibody (H-7) an invaluable tool for researchers studying exocytosis and related cellular mechanisms.

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Unc18-3 Antibody (H-7) References:

1. Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis.  |  Barclay, JW., et al. 2003. J Biol Chem. 278: 10538-45. PMID: 12519779
2. A role for Sec1/Munc18 proteins in platelet exocytosis.  |  Schraw, TD., et al. 2003. Biochem J. 374: 207-17. PMID: 12773094
3. Alcoholic chronic pancreatitis involves displacement of Munc18c from the pancreatic acinar basal membrane surface.  |  Gaisano, HY., et al. 2004. Pancreas. 28: 395-400. PMID: 15097857
4. Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis.  |  Graham, ME., et al. 2004. J Biol Chem. 279: 32751-60. PMID: 15175344
5. Regulation of syntaxin1A-munc18 complex for SNARE pairing in HEK293 cells.  |  Gladycheva, SE., et al. 2004. J Physiol. 558: 857-71. PMID: 15218059
6. Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo.  |  Liu, J., et al. 2004. J Biol Chem. 279: 55924-36. PMID: 15489225
7. Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.  |  Ciufo, LF., et al. 2005. Mol Biol Cell. 16: 470-82. PMID: 15563604
8. Protease-activated receptor-1 activation of endothelial cells induces protein kinase Calpha-dependent phosphorylation of syntaxin 4 and Munc18c: role in signaling p-selectin expression.  |  Fu, J., et al. 2005. J Biol Chem. 280: 3178-84. PMID: 15576373
9. Expression and transcriptional regulation of Munc18 isoforms in mast cells.  |  Nigam, R., et al. 2005. Biochim Biophys Acta. 1728: 77-83. PMID: 15777698

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Unc18-3 Antibody (H-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Unc18-3 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Unc18-3 Antibody (H-7) Specifications

Alternate Product Name: Unc18-3

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 501-533

Epitope Target: Unc18-3

Epitope Species: human

Product Reactivity/Detection: Unc18-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Unc18-3 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Unc18-1/2 antibody is Unc18-1/2 Antibody (D-1): sc-133169.