UKHC Antibody (F-9): sc-133185

UKHC Antibody (F-9) is a mouse monoclonal IgG1 antibody that detects ubiquitous kinesin heavy chain (UKHC) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UKHC is a vital kinesin motor protein that plays a crucial role in intracellular transport, facilitating the movement of organelles, vesicles, and other cargo along microtubules, which is essential for maintaining cellular function and organization. UKHC (F-9) antibody recognizes a protein structure characterized by three distinct domains: a large globular N-terminal domain that regulates ATP hydrolysis and microtubule binding, central coiled-coil domains that promote dimerization of the heavy chains, and small globular C-terminal domains that interact with kinesin light chains and various membranous organelles. This structural organization allows UKHC to effectively transport cargo within the cell, particularly in areas of high vesicular trafficking, such as the microtubule pathways in neural cells and the Golgi apparatus in non-neural cell types. The ability of UKHC to mediate these transport processes is critical for numerous cellular activities, including neurotransmitter release, organelle positioning, and overall cellular homeostasis. Anti-UKHC antibody (F-9) serves as an invaluable tool for researchers studying the dynamics of intracellular transport and the role of kinesin in various physiological and pathological contexts.

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UKHC Antibody (F-9) References:

1. Defective kinesin heavy chain behavior in mouse kinesin light chain mutants.  |  Rahman, A., et al. 1999. J Cell Biol. 146: 1277-88. PMID: 10491391
2. The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23.  |  Diefenbach, RJ., et al. 2002. Biochemistry. 41: 14906-15. PMID: 12475239
3. Beta-dystrobrevin interacts directly with kinesin heavy chain in brain.  |  Macioce, P., et al. 2003. J Cell Sci. 116: 4847-56. PMID: 14600269
4. The ribosome receptor, p180, interacts with kinesin heavy chain, KIF5B.  |  Diefenbach, RJ., et al. 2004. Biochem Biophys Res Commun. 319: 987-92. PMID: 15184079
5. Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport.  |  Gupta, V., et al. 2008. Traffic. 9: 1850-66. PMID: 18817524
6. Reduction of kinesin I heavy chain decreases tau hyperphosphorylation, aggregation, and memory impairment in Alzheimer's disease and tauopathy models.  |  Selvarasu, K., et al. 2022. Front Mol Biosci. 9: 1050768. PMID: 36387285
7. Interaction of Tau with Kinesin-1: Effect of Kinesin-1 Heavy Chain Elimination on Autophagy-Mediated Mutant Tau Degradation.  |  Selvarasu, K., et al. 2023. Biomedicines. 12: PMID: 38275365
8. Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria.  |  Tanaka, Y., et al. 1998. Cell. 93: 1147-58. PMID: 9657148
9. Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response.  |  Axenovich, SA., et al. 1998. Cancer Res. 58: 3423-8. PMID: 9699675
10. Chromosomal localization reveals three kinesin heavy chain genes in mouse.  |  Xia, Ch., et al. 1998. Genomics. 52: 209-13. PMID: 9782088

The preferred UKHC antibody is UKHC Antibody (F-5): sc-133184.