UKHC Antibody (F-5): sc-133184

UKHC Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects UKHC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). UKHC (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UKHC protein, also known as ubiquitous kinesin heavy chain, plays a crucial role in intracellular transport, facilitating the movement of organelles and vesicles along microtubules, which is essential for maintaining cellular organization and function. UKHC protein is particularly important in neurons, where UKHC supports the transport of synaptic vesicles and other critical components necessary for neurotransmission. Structurally, kinesin proteins, including UKHC, are characterized by three distinct domains: a large globular N-terminal domain that regulates ATP hydrolysis and microtubule binding, central coiled-coil domains that promote dimerization of heavy chains, and small globular C-terminal domains that interact with kinesin light chains and various membranous organelles. UKHC expression is predominantly localized in areas of active vesicular trafficking, such as the microtubule pathways in neural cells and the Golgi apparatus in non-neural cell types, highlighting UKHC′s vital role in cellular transport mechanisms.

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UKHC Antibody (F-5) References:

1. Purification and characterization of native conventional kinesin, HSET, and CENP-E from mitotic hela cells.  |  DeLuca, JG., et al. 2001. J Biol Chem. 276: 28014-21. PMID: 11382767
2. Herpes simplex virus tegument protein US11 interacts with conventional kinesin heavy chain.  |  Diefenbach, RJ., et al. 2002. J Virol. 76: 3282-91. PMID: 11884553
3. Evidence of kinesin heavy chain (KIF5A) involvement in pure hereditary spastic paraplegia.  |  Fichera, M., et al. 2004. Neurology. 63: 1108-10. PMID: 15452312
4. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent.  |  Glater, EE., et al. 2006. J Cell Biol. 173: 545-57. PMID: 16717129
5. A family-based association study of kinesin heavy chain member 2 gene (KIF2) and schizophrenia.  |  Li, C., et al. 2006. Neurosci Lett. 407: 151-5. PMID: 16959419
6. Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.  |  Wright, BD., et al. 1991. J Cell Biol. 113: 817-33. PMID: 1827446
7. Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport.  |  Gupta, V., et al. 2008. Traffic. 9: 1850-66. PMID: 18817524
8. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.  |  Ingold, AL., et al. 1988. J Cell Biol. 107: 2657-67. PMID: 2974459
9. Association of kinesin with the Golgi apparatus in rat hepatocytes.  |  Marks, DL., et al. 1994. J Cell Sci. 107 (Pt 9): 2417-26. PMID: 7844161
10. Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.  |  Bi, GQ., et al. 1997. J Cell Biol. 138: 999-1008. PMID: 9281579
11. Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria.  |  Tanaka, Y., et al. 1998. Cell. 93: 1147-58. PMID: 9657148
12. Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response.  |  Axenovich, SA., et al. 1998. Cancer Res. 58: 3423-8. PMID: 9699675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UKHC Antibody (F-5) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-UKHC Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UKHC Antibody (F-5) Specifications

Alternate Product Name: UKHC

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 691-740

Epitope Target: UKHC

Epitope Species: human

Product Reactivity/Detection: ubiquitous kinesin heavy chain (UKHC)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for UKHC Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin