UFD2 Antibody (RQ-5): sc-100610
- UFD2 Antibody (RQ-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 100 µg/ml
- raised against recombinant UFD2 of human origin
- recommended for detection of UFD2 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UFD2 Antibody (RQ-5). This work is in progress.
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UFD2 Antibody (RQ-5) is a mouse monoclonal IgG1 antibody that detects UFD2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). UFD2, also known as ubiquitin conjugation factor E4 (UBE4B), plays a crucial role in the ubiquitin-proteasome system by facilitating the assembly of polyubiquitin chains, which are essential for targeting proteins for degradation. This process is vital for maintaining cellular homeostasis, regulating protein turnover, and responding to cellular stress. Notably, UFD2 is proteolytically cleaved during apoptosis, with caspase-6 and granzyme B being the most efficient in cleaving at Asp 123, which highlights its involvement in programmed cell death. The highest expression levels of UFD2 are found in the ovary, testis, heart, and skeletal muscle, indicating its importance in reproductive and muscular functions. Anti-UFD2 antibody (RQ-5) is an invaluable tool for researchers studying the dynamics of protein degradation and the implications of UFD2 in various physiological and pathological processes.
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UFD2 Antibody (RQ-5) References:
- Screening for gene mutations in a 500 kb neuroblastoma tumor suppressor candidate region in chromosome 1p; mutation and stage-specific expression in UBE4B/UFD2. | Krona, C., et al. 2003. Oncogene. 22: 2343-51. PMID: 12700669
- Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage. | Saeki, Y., et al. 2004. Biochem Biophys Res Commun. 320: 840-5. PMID: 15240124
- Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation. | Spinette, S., et al. 2004. Cell Cycle. 3: 1638-44. PMID: 15611659
- Cdc48-Ufd2-Rad23: the road less ubiquitinated? | Bazirgan, OA. and Hampton, RY. 2005. Nat Cell Biol. 7: 207-9. PMID: 15738968
- The two human homologues of yeast UFD2 ubiquitination factor, UBE4A and UBE4B, are located in common neuroblastoma deletion regions and are subject to mutations in tumours. | Carén, H., et al. 2006. Eur J Cancer. 42: 381-7. PMID: 16386891
- Cellular functions of Ufd2 and Ufd3 in proteasomal protein degradation depend on Cdc48 binding. | Böhm, S., et al. 2011. Mol Cell Biol. 31: 1528-39. PMID: 21282470
- Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase. | Liu, C., et al. 2011. J Biol Chem. 286: 43660-43667. PMID: 22045814
- E4-Ubiquitin ligase Ufd2 stabilizes Yap8 and modulates arsenic stress responses independent of the U-box motif. | Ferreira, RT., et al. 2015. Biol Open. 4: 1122-31. PMID: 26276098
- A heterotypic assembly mechanism regulates CHIP E3 ligase activity. | Das, A., et al. 2022. EMBO J. 41: e109566. PMID: 35762422
- E4 ubiquitin ligase promotes mitofusin turnover and mitochondrial stress response. | Anton, V., et al. 2023. Mol Cell. 83: 2976-2990.e9. PMID: 37595558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD2 Antibody (RQ-5) | sc-100610 | 100 µg/ml | $339.00 |