Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UFD1 CRISPR/Cas9 KO Plasmid (m): sc-423598
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UFD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UFD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UFD1 Antibody (B-7): sc-377265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD1 CRISPR/Cas9 KO Plasmid (m) | sc-423598 | 20 µg | $397.00 |
Overview
Ufd1l encodes UFD1, a core component of the UFD1–NPL4–VCP/p97 segregase complex that recognizes and extracts polyubiquitinated substrates for downstream processing by the ubiquitin–proteasome system. Through coupling ubiquitin signaling to ATP-dependent protein remodeling, UFD1 supports ER-associated degradation (ERAD), quality control of misfolded proteins, and proteostasis under cellular stress. This pathway also interfaces with cell cycle progression, DNA damage responses, and regulation of transcriptional programs via controlled turnover of key regulators. Dysregulation of p97 cofactors and ERAD components has been linked to proteotoxic stress phenotypes and neurodegenerative and developmental biology contexts, making Ufd1l a useful node for mechanistic studies in mouse models.
UFD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ufd1l gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ufd1l together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ufd1l open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UFD1 protein expression.
This CRISPR knockout system enables efficient generation of Ufd1l-deficient cell models for investigation of UFD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ufd1l exon(s) critical for UFD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ufd1l genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UFD1 CRISPR/Cas9 KO Plasmid (m) and UFD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ufd1l locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UFD1 HDR Plasmid (m) and UFD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ufd1l homology arms to support homology-directed repair at defined Ufd1l target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.