UFD1 Antibody (B-7): sc-377265

UFD1 Antibody (B-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects UFD1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UFD1 (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UFD1 plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. Specifically, UFD1 is involved in the ubiquitin fusion degradation pathway, where UFD1 helps to manage the degradation of ubiquitin-conjugated proteins that have undergone specific post-translational modifications. These modifications are vital for the recognition and processing of proteins destined for degradation, ensuring that damaged or misfolded proteins do not accumulate within the cell. The identification of UFD1 in yeast and subsequent homologs in humans highlights evolutionary conservation and importance in cellular function. Mutations in the UFD1 gene have been linked to CATCH22 syndrome, a condition that underscores UFD1′s significance in developmental processes, particularly in cardiac and craniofacial development.

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UFD1 Antibody (B-7) References:

1. The ubiquitin-conjugation system.  |  Jentsch, S. 1992. Annu Rev Genet. 26: 179-207. PMID: 1336336
2. The Cdc48/p97-Ufd1-Npl4 complex: its potential role in coordinating cellular morphogenesis during the M-G1 transition.  |  Cao, K. and Zheng, Y. 2004. Cell Cycle. 3: 422-4. PMID: 15004522
3. Ssz1 restores endoplasmic reticulum-associated protein degradation in cells expressing defective cdc48-ufd1-npl4 complex by upregulating cdc48.  |  Bosis, E., et al. 2010. Genetics. 184: 695-706. PMID: 20038635
4. SUMO enhances unfolding of SUMO-polyubiquitin-modified substrates by the Ufd1/Npl4/Cdc48 complex.  |  Lee, HG., et al. 2023. Proc Natl Acad Sci U S A. 120: e2213703120. PMID: 36574706
5. VCF1 is a p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates.  |  Mirsanaye, AS., et al. 2024. Nat Commun. 15: 2459. PMID: 38503733
6. Trim21 modulates endoplasmic reticulum-associated degradation and sensitizes cancer cells to ER stress-induced apoptosis by inhibiting VCP/Npl4/UFD1 assembly.  |  Yuan, C., et al. 2025. Biochim Biophys Acta Mol Basis Dis. 1871: 167533. PMID: 39368714
7. Targeting DTX2/UFD1-mediated FTO degradation to regulate antitumor immunity.  |  Cui, YH., et al. 2024. Proc Natl Acad Sci U S A. 121: e2407910121. PMID: 39661064
8. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
9. A proteolytic pathway that recognizes ubiquitin as a degradation signal.  |  Johnson, ES., et al. 1995. J Biol Chem. 270: 17442-56. PMID: 7615550
10. UFD1L, a developmentally expressed ubiquitination gene, is deleted in CATCH 22 syndrome.  |  Pizzuti, A., et al. 1997. Hum Mol Genet. 6: 259-65. PMID: 9063746
11. Pathways of ubiquitin conjugation.  |  Haas, AL. and Siepmann, TJ. 1997. FASEB J. 11: 1257-68. PMID: 9409544
12. Structure and expression of the human ubiquitin fusion-degradation gene (UFD1L).  |  Novelli, G., et al. 1998. Biochim Biophys Acta. 1396: 158-62. PMID: 9540831

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UFD1 Antibody (B-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-UFD1 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UFD1 Antibody (B-7) Specifications

Alternate Product Name: UFD1

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 181-307

Epitope Target: UFD1

Epitope Species: human

Product Reactivity/Detection: UFD1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for UFD1 Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin