UFD1 Antibody (E-9): sc-377222
- UFD1 Antibody (E-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 181-307 mapping at the C-terminus of UFD1 of human origin
- recommended for detection of UFD1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UFD1 Antibody (E-9) for WB applications. These reagents are now offered in bundles with UFD1 Antibody (E-9) (see ordering information below).
QUICK LINKS
UFD1 Antibody (E-9) is a mouse monoclonal IgG1 antibody that detects UFD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UFD1 plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. UFD1 is primarily located in the cytoplasm and nucleus, where UFD1 participates in the recognition and processing of ubiquitin-conjugated substrates, thereby influencing the degradation of misfolded or damaged proteins. Proper functioning of UFD1 is vital, as dysregulation can lead to the accumulation of harmful proteins, contributing to various diseases, including neurodegenerative disorders. Notably, mutations in the UFD1 gene have been linked to CATCH22 syndrome, underscoring UFD1′s importance in developmental processes and potential implications in human health. Anti-UFD1 antibody (E-9) is an invaluable tool for researchers studying the dynamics of protein degradation and the role of ubiquitin-mediated pathways in cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UFD1 Antibody (E-9) References:
- A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. | Meyer, HH., et al. 2000. EMBO J. 19: 2181-92. PMID: 10811609
- Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. | Rape, M., et al. 2001. Cell. 107: 667-77. PMID: 11733065
- Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4. | Meyer, HH., et al. 2002. EMBO J. 21: 5645-52. PMID: 12411482
- Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains. | Ye, Y., et al. 2003. J Cell Biol. 162: 71-84. PMID: 12847084
- The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism. | Bruderer, RM., et al. 2004. J Biol Chem. 279: 49609-16. PMID: 15371428
- Ufd1 exhibits dual ubiquitin binding modes. | Walters, KJ. 2005. Structure. 13: 943-4. PMID: 16004865
- Detailed structural insights into the p97-Npl4-Ufd1 interface. | Isaacson, RL., et al. 2007. J Biol Chem. 282: 21361-9. PMID: 17491009
- Analysis of Npl4 deletion mutants in mammalian cells unravels new Ufd1-interacting motifs and suggests a regulatory role of Npl4 in ERAD. | Lass, A., et al. 2008. Exp Cell Res. 314: 2715-23. PMID: 18586029
- The Cdc48-Ufd1-Npl4 complex is central in ubiquitin-proteasome triggered catabolite degradation of fructose-1,6-bisphosphatase. | Barbin, L., et al. 2010. Biochem Biophys Res Commun. 394: 335-41. PMID: 20206597
- Evaluating p97 Inhibitor Analogues for Potency against p97-p37 and p97-Npl4-Ufd1 Complexes. | Gui, L., et al. 2016. ChemMedChem. 11: 953-7. PMID: 27043824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD1 Antibody (E-9) | sc-377222 | 200 µg/ml | $322.00 | |||
UFD1 Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-538027 | 200 µg Ab; 10 µg BP | $361.00 | |||
UFD1 Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-535548 | 200 µg Ab; 40 µg BP | $361.00 | |||
UFD1 Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-545493 | 200 µg Ab; 20 µg BP | $361.00 |