UCP4 Antibody (A-5): sc-365295

UCP4 Antibody (A-5) is a mouse monoclonal IgM antibody that detects UCP4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-UCP4 antibody (A-5) is available as the non-conjugated form. UCP4, also known as SLC25A27, is a member of the uncoupling protein family, which plays a crucial role in thermoregulation and energy metabolism. UCP4 is primarily located in the inner mitochondrial membrane, where UCP4 facilitates proton transport, dissipating the proton gradient and generating heat instead of ATP. This function is particularly important in thermogenic tissues, such as brown adipose tissue, where UCPs help regulate metabolic rate and energy expenditure. UCP4 is unique among its family members due to expression in both fetal and adult brain tissues, indicating potential involvement in brain thermoregulation and metabolic adaptation. UCP4 mRNA levels increase in response to acute cold exposure, suggesting a role in the brain′s response to environmental changes. UCP4 consists of 323 amino acids and features specific sequences in its alpha-helices that are critical for fatty acid anion binding and translocation, further underscoring importance in metabolic processes.

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UCP4 Antibody (A-5) References:

1. UCP4, a novel brain-specific mitochondrial protein that reduces membrane potential in mammalian cells.  |  Mao, W., et al. 1999. FEBS Lett. 443: 326-30. PMID: 10025957
2. Uncoupling protein homologs: emerging views of physiological function.  |  Adams, SH. 2000. J Nutr. 130: 711-4. PMID: 10736318
3. Specific sequence of motifs of mitochondrial uncoupling proteins.  |  Jezek, P. and Urbánková, E. 2000. IUBMB Life. 49: 63-70. PMID: 10772343
4. Characterization of novel UCP5/BMCP1 isoforms and differential regulation of UCP4 and UCP5 expression through dietary or temperature manipulation.  |  Yu, XX., et al. 2000. FASEB J. 14: 1611-8. PMID: 10928996
5. Mitochondrial uncoupling proteins and phylogenesis--UCP4 as the ancestral uncoupling protein.  |  Hanák, P. and Jezek, P. 2001. FEBS Lett. 495: 137-41. PMID: 11334880
6. The role of uncoupling proteins in pathophysiological states.  |  Argilés, JM., et al. 2002. Biochem Biophys Res Commun. 293: 1145-52. PMID: 12054495
7. Mitochondrial potassium channels and uncoupling proteins in synaptic plasticity and neuronal cell death.  |  Mattson, MP. and Liu, D. 2003. Biochem Biophys Res Commun. 304: 539-49. PMID: 12729589
8. Evolution of mitochondrial uncoupling proteins: novel invertebrate UCP homologues suggest early evolutionary divergence of the UCP family.  |  Sokolova, IM. and Sokolov, EP. 2005. FEBS Lett. 579: 313-7. PMID: 15642337
9. Transcriptional regulation of UCP4 by NF-kappaB and its role in mediating protection against MPP+ toxicity.  |  Ho, JW., et al. 2010. Free Radic Biol Med. 49: 192-204. PMID: 20385226
10. UCP4 overexpression improves fatty acid oxidation and insulin sensitivity in L6 myocytes.  |  Gao, CL., et al. 2011. J Bioenerg Biomembr. 43: 109-18. PMID: 21607879
11. Uncoupling protein-4 (UCP4) increases ATP supply by interacting with mitochondrial Complex II in neuroblastoma cells.  |  Ho, PW., et al. 2012. PLoS One. 7: e32810. PMID: 22427795
12. Ucp4 Knockdown of Cerebellar Purkinje Cells Induces Bradykinesia.  |  Wang, YY., et al. 2024. Mol Neurobiol. 61: 1119-1139. PMID: 37688710

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UCP4 Antibody (A-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-UCP4 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UCP4 Antibody (A-5) Specifications

Alternate Product Name: UCP4

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 17-37

Epitope Target: UCP4

Epitope Species: human

Product Reactivity/Detection: UCP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for UCP4 Antibody (A-5): P: Blocking Peptide