Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UCK1 CRISPR/Cas9 KO Plasmid (m): sc-423608
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UCK1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UCK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UCK1 Antibody (E-9): sc-373940
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCK1 CRISPR/Cas9 KO Plasmid (m) | sc-423608 | 20 µg | $397.00 |
Overview
Uck1 encodes uridine-cytidine kinase 1 (UCK1), a cytosolic salvage-pathway enzyme that phosphorylates uridine and cytidine to UMP and CMP, supplying pyrimidine nucleotides for RNA synthesis, DNA replication, and membrane phospholipid metabolism. By regulating nucleotide pool balance, UCK1 influences proliferative capacity, cell-cycle progression, and stress responses linked to replication and transcriptional demand. In mouse systems, UCK1 activity is commonly examined alongside de novo pyrimidine synthesis and nucleoside transport to understand metabolic rewiring and compensatory flux within nucleotide homeostasis. Altered pyrimidine metabolism is a recurring feature of disorders involving dysregulated proliferation and genome maintenance, making Uck1 a useful node for pathway-focused mechanistic studies.
UCK1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Uck1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Uck1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Uck1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UCK1 protein expression.
This CRISPR knockout system enables efficient generation of Uck1-deficient cell models for investigation of UCK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Uck1 exon(s) critical for UCK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Uck1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UCK1 CRISPR/Cas9 KO Plasmid (m) and UCK1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Uck1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UCK1 HDR Plasmid (m) and UCK1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Uck1 homology arms to support homology-directed repair at defined Uck1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.