UBPY Antibody (F-3): sc-365481

UBPY Antibody (F-3) is a mouse monoclonal IgG1 antibody that detects UBPY in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBPY, also known as USP8 (ubiquitin carboxyl-terminal hydrolase 8) or KIAA0055, is a crucial 1,118 amino acid protein that plays a significant role in the ubiquitin pathway, which is essential for regulating various intracellular processes, including signal transduction, transcriptional activation, and cell cycle progression. UBPY is primarily located in the cytoplasm and nucleus, where UBPY participates in the dynamic regulation of protein turnover and cellular responses. UBPY′s ability to remove ubiquitin from ubiquitin-conjugated proteins is vital for maintaining cellular homeostasis and modulating T-cell anergy, which is critical for immune response regulation. UBPY exists in a ternary complex with OTUB1 and GRAIL, highlighting UBPY′s importance in the intricate network of ubiquitin signaling. The gene encoding UBPY is located on human chromosome 15, a region that contains over 700 genes and represents nearly 3% of the human genome, underscoring UBPY′s evolutionary significance and potential implications in various biological processes. Anti-UBPY antibody (F-3) is an invaluable tool for researchers investigating the complexities of ubiquitin-mediated signaling and its impact on cellular functions.

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UBPY Antibody (F-3) References:

1. CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis.  |  Crespo-Yàñez, X., et al. 2018. PLoS Genet. 14: e1007456. PMID: 29933386
2. The ubiquitin-specific protease USP8 directly deubiquitinates SQSTM1/p62 to suppress its autophagic activity.  |  Peng, H., et al. 2020. Autophagy. 16: 698-708. PMID: 31241013
3. The METTL3/MALAT1/PTBP1/USP8/TAK1 axis promotes pyroptosis and M1 polarization of macrophages and contributes to liver fibrosis.  |  Shu, B., et al. 2021. Cell Death Discov. 7: 368. PMID: 34839365
4. USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.  |  Xiong, W., et al. 2022. Nat Commun. 13: 1700. PMID: 35361799
5. USP8 promotes cancer progression and extracellular vesicle-mediated CD8+ T cell exhaustion by deubiquitinating the TGF-β receptor TβRII.  |  Xie, F., et al. 2022. EMBO J. 41: e108791. PMID: 35811497
6. USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization.  |  Tang, J., et al. 2023. Cell Death Dis. 14: 360. PMID: 37311739
7. Targeting USP8 Inhibits O-GlcNAcylation of SLC7A11 to Promote Ferroptosis of Hepatocellular Carcinoma via Stabilization of OGT.  |  Tang, J., et al. 2023. Adv Sci (Weinh). 10: e2302953. PMID: 37867237
8. USP8 prevents aberrant NF-κB and Nrf2 activation by counteracting ubiquitin signals from endosomes.  |  Endo, A., et al. 2024. J Cell Biol. 223: PMID: 38180476
9. USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.  |  Li, H., et al. 2024. Proc Natl Acad Sci U S A. 121: e2315541121. PMID: 38598341
10. Stress granule-localized USP8 potentiates cGAS-mediated type I interferonopathies through deubiquitination of DDX3X.  |  Zhang, X., et al. 2024. Cell Rep. 43: 114248. PMID: 38795350

The preferred UBPY antibody is UBPY Antibody (E-1): sc-376130.