Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ubiquitin CRISPR/Cas9 KO Plasmid (h): sc-400526
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ubiquitin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ubiquitin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ubiquitin Antibody (P4D1): sc-8017
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ubiquitin CRISPR/Cas9 KO Plasmid (h) | sc-400526 | 20 µg | $397.00 |
Overview
Human UBB encodes ubiquitin, a highly conserved 76–amino acid protein that is covalently attached to substrates to regulate protein fate and signaling. Ubiquitination controls proteasomal degradation and non-proteolytic signaling through K48- and K63-linked chains, shaping pathways that include protein quality control, DNA damage responses, cell cycle progression, endocytosis, and innate immune signaling. Ubiquitin homeostasis and ubiquitin-dependent proteostasis are closely tied to cellular stress responses, with dysregulation frequently implicated in neurodegeneration, cancer biology, and inflammatory phenotypes. Because UBB contributes to the cellular ubiquitin pool, perturbation of its expression can influence global ubiquitin availability and downstream ubiquitin–proteasome system dynamics.
Ubiquitin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ubiquitin protein expression.
This CRISPR knockout system enables efficient generation of UBB-deficient cell models for investigation of Ubiquitin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBB exon(s) critical for Ubiquitin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ubiquitin CRISPR/Cas9 KO Plasmid (h) and Ubiquitin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ubiquitin HDR Plasmid (h) and Ubiquitin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBB homology arms to support homology-directed repair at defined UBB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.