Ubiquitin Antibody (P4G7): sc-53509

Ubiquitin Antibody (P4G7) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect full-length denatured Ubiquitin protein of bovine origin. Anti-Ubiquitin antibody (P4G7) recognizes Ubiquitin from mouse, rat, human, bovine, and yeast sources across a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and multiple conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Ubiquitin monoclonal antibody (P4G7) offers versatile solutions to meet diverse experimental requirements. Ubiquitin is one of the most evolutionarily conserved proteins, ubiquitously localized within various cellular compartments including the cytoplasm, nucleus, and mitochondria, which is crucial for maintaining cellular homeostasis. Ubiquitin′s primary function involves tagging abnormal, foreign, and improperly folded proteins for degradation by the 26S Proteasome, a process essential for protein quality control and regulation of numerous cellular pathways. This tagging is achieved through the covalent attachment of Ubiquitin to substrate proteins via an isopeptide bond between its carboxy-terminal glycine and lysine residues on target proteins, enabling the formation of polyubiquitin chains, particularly through Lysine 48 linkages that signal for proteolysis. Beyond protein degradation, Ubiquitin plays a vital role in regulating signal transduction pathways, DNA repair, and cell cycle progression by modulating the stability and function of key regulatory proteins such as IκB-α and p27. Ubiquitin also contributes to endocytosis and receptor trafficking, expanding its role beyond intracellular protein turnover. Strategic localization of Ubiquitin within different cellular compartments ensures efficient regulation of these processes, highlighting Ubiquitin (P4G7) antibody as an essential tool for researchers studying protein turnover, cellular signaling mechanisms, and the intricate networks governing cellular regulation.

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Ubiquitin Antibody (P4G7) References:

1. Kinetic analysis of the conjugation of ubiquitin to picornavirus 3C proteases catalyzed by the mammalian ubiquitin-protein ligase E3alpha.  |  Lawson, TG., et al. 2001. J Biol Chem. 276: 39629-37. PMID: 11526102
2. Ubiquitin protein ligase Nedd4 binds to connexin43 by a phosphorylation-modulated process.  |  Leykauf, K., et al. 2006. J Cell Sci. 119: 3634-42. PMID: 16931598
3. Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export.  |  Woo, JL. and Berk, AJ. 2007. J Virol. 81: 575-87. PMID: 17079297
4. Multiple forms of ubiquitin-protein ligase. Binding of activated ubiquitin to protein substrates.  |  Lee, PL., et al. 1986. Biochemistry. 25: 3134-8. PMID: 3015198
5. Alterations in components of the ubiquitin-protein ligase system following maturation of reticulocytes to erythrocytes.  |  Raviv, O., et al. 1987. Biochem Biophys Res Commun. 145: 658-65. PMID: 3593363
6. Ubiquitin-mediated processing of NF-kappa B transcriptional activator precursor p105. Reconstitution of a cell-free system and identification of the ubiquitin-carrier protein, E2, and a novel ubiquitin-protein ligase, E3, involved in conjugation.  |  Orian, A., et al. 1995. J Biol Chem. 270: 21707-14. PMID: 7665588
7. The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53.  |  Scheffner, M., et al. 1993. Cell. 75: 495-505. PMID: 8221889
8. Ubiquitination mediated by the Npi1p/Rsp5p ubiquitin-protein ligase is required for endocytosis of the yeast uracil permease.  |  Galan, JM., et al. 1996. J Biol Chem. 271: 10946-52. PMID: 8631913
9. Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases.  |  Hatakeyama, S., et al. 1997. J Biol Chem. 272: 15085-92. PMID: 9182527
10. Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes.  |  Anan, T., et al. 1998. Genes Cells. 3: 751-63. PMID: 9990509

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ubiquitin Antibody (P4G7) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-Ubiquitin Antibody (P4G7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ubiquitin Antibody (P4G7) Specifications

Alternate Product Name: Ubiquitin

Product Clone Name: P4G7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Ubiquitin

Epitope Species: bovine

Product Reactivity/Detection: Ubiquitin, polyubiquitin and Ubiquitin-conjugated proteins

Species Reactivity/Detection: mouse, rat, human, bovine, yeast

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for Ubiquitin Antibody (P4G7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Ubiquitin antibody is Ubiquitin Antibody (P4D1): sc-8017.