Ubiquitin Antibody (F-11): sc-271289
- Ubiquitin Antibody (F-11) is a mouse monoclonal IgG1 κ, cited in 72 publications, provided at 200 µg/ml
- raised against amino acids 1-76 representing full length Ubiquitin of human origin
- Ubiquitin Antibody (F-11) is recommended for detection of Ubiquitin and polyubiquitin of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Ubiquitin Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Ubiquitin Antibody (F-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with Ubiquitin Antibody (F-11) (see ordering information below).
Ubiquitin Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect full-length human Ubiquitin protein, encompassing amino acids 1-76. Ubiquitin monoclonal antibody (F-11) recognizes Ubiquitin from mouse, rat, human, avian, as well as additional species including equine, canine, bovine, and porcine origins across applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and multiple conjugated variants including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-Ubiquitin antibody (F-11) provides versatile solutions tailored to diverse experimental needs. The primary post-translational modification of Ubiquitin is the formation of isopeptide bonds with lysine residues on target proteins, a process known as ubiquitination. This modification can result in single Ubiquitin molecule attachment (monoubiquitination) or polyubiquitin chain formation through different lysine linkages, such as Lysine 48 (K48) and Lysine 63 (K63). K48-linked polyubiquitination typically signals for proteasomal degradation via the 26S Proteasome, thereby regulating protein turnover and maintaining protein quality control within cells. K63-linked polyubiquitination participates in non-proteolytic functions such as DNA repair, signal transduction, and endocytosis, highlighting Ubiquitin′s versatility in cellular processes. Ubiquitin′s ability to engage in diverse post-translational modifications proves crucial for regulating various cellular pathways, including degradation of abnormal, foreign, and improperly folded proteins, as well as modulation of key regulatory proteins such as IκB-α and p27 involved in signal transduction and cell cycle progression. Ubiquitin (F-11) antibody serves as an essential tool for investigating Ubiquitin′s diverse roles in protein regulation, signal transduction, and cellular homeostasis across multiple species and experimental applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ubiquitin Antibody (F-11) | sc-271289 | 200 µg/ml | $322.00 | |||
Ubiquitin Antibody (F-11): m-IgG Fc BP-HRP Bundle | sc-525795 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ubiquitin Antibody (F-11) AC | sc-271289 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ubiquitin Antibody (F-11) HRP | sc-271289 HRP | 200 µg/ml | $322.00 | |||
Ubiquitin Antibody (F-11) FITC | sc-271289 FITC | 200 µg/ml | $336.00 | |||
Ubiquitin Antibody (F-11) PE | sc-271289 PE | 200 µg/ml | $349.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 488 | sc-271289 AF488 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 546 | sc-271289 AF546 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 594 | sc-271289 AF594 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 647 | sc-271289 AF647 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 680 | sc-271289 AF680 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (F-11) Alexa Fluor® 790 | sc-271289 AF790 | 200 µg/ml | $364.00 |