UBE2J1 Antibody (B-6): sc-377002
- UBE2J1 Antibody (B-6) is a mouse monoclonal IgG1 κ UBE2J1 antibody, cited in 14 publications, provided at 200 µg/ml
- raised against amino acids 93-196 mapping within an internal region of UBE2J1 of human origin
- UBE2J1 Antibody (B-6) is recommended for detection of UBE2J1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- Anti-UBE2J1 Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE2J1 Antibody (B-6) for WB and IHC(P) applications. These reagents are now offered in bundles with UBE2J1 Antibody (B-6) (see ordering information below).
UBE2J1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBE2J1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UBE2J1 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE2J1, also known as Ubiquitin-conjugating enzyme E2 J1, Ubc6p, CGI-76, NCUBE1, HSPC153, or HSPC205, is a 318 amino acid single-pass type IV membrane protein that plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis. UBE2J1 is primarily localized to the membrane of the endoplasmic reticulum (ER), where UBE2J1 catalyzes the attachment of ubiquitin to misfolded membrane proteins, thereby marking them for proteasomal degradation. This process is vital for quality control within the ER, as UBE2J1 ensures that only properly folded proteins proceed through the secretory pathway, preventing the accumulation of potentially toxic misfolded proteins that could disrupt cellular function. The ATP-dependent reaction facilitated by UBE2J1 results in the production of AMP, a diphosphate, and a ubiquitin-tagged protein, highlighting UBE2J1′s importance in cellular protein management and overall cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE2J1 Antibody (B-6) References:
- Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. | Lester, D., et al. 2000. Biochem Biophys Res Commun. 269: 474-80. PMID: 10708578
- Degradation signals recognized by the Ubc6p-Ubc7p ubiquitin-conjugating enzyme pair. | Gilon, T., et al. 2000. Mol Cell Biol. 20: 7214-9. PMID: 10982838
- Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s). | Tiwari, S. and Weissman, AM. 2001. J Biol Chem. 276: 16193-200. PMID: 11278356
- Sec61p-independent degradation of the tail-anchored ER membrane protein Ubc6p. | Walter, J., et al. 2001. EMBO J. 20: 3124-31. PMID: 11406589
- A role for mammalian Ubc6 homologues in ER-associated protein degradation. | Lenk, U., et al. 2002. J Cell Sci. 115: 3007-14. PMID: 12082160
- Ubc6p and ubc7p are required for normal and substrate-induced endoplasmic reticulum-associated degradation of the human selenoprotein type 2 iodothyronine monodeiodinase. | Botero, D., et al. 2002. Mol Endocrinol. 16: 1999-2007. PMID: 12198238
- TNF-alpha induced c-IAP1/TRAF2 complex translocation to a Ubc6-containing compartment and TRAF2 ubiquitination. | Wu, CJ., et al. 2005. EMBO J. 24: 1886-98. PMID: 15861135
- Human homologs of Ubc6p ubiquitin-conjugating enzyme and phosphorylation of HsUbc6e in response to endoplasmic reticulum stress. | Oh, RS., et al. 2006. J Biol Chem. 281: 21480-21490. PMID: 16720581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2J1 Antibody (B-6) | sc-377002 | 200 µg/ml | $316.00 | |||
UBE2J1 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-529954 | 200 µg Ab; 10 µg BP | $354.00 | |||
UBE2J1 Antibody (B-6): m-IgGκ BP-HRP Bundle | sc-523147 | 200 µg Ab, 40 µg BP | $354.00 | |||
UBE2J1 Antibody (B-6): m-IgG1 BP-HRP Bundle | sc-543784 | 200 µg Ab; 20 µg BP | $354.00 | |||
UBE2J1 Antibody (B-6) AC | sc-377002 AC | 500 µg/ml, 25% agarose | $416.00 | |||
UBE2J1 Antibody (B-6) HRP | sc-377002 HRP | 200 µg/ml | $316.00 | |||
UBE2J1 Antibody (B-6) FITC | sc-377002 FITC | 200 µg/ml | $330.00 | |||
UBE2J1 Antibody (B-6) PE | sc-377002 PE | 200 µg/ml | $343.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 488 | sc-377002 AF488 | 200 µg/ml | $357.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 546 | sc-377002 AF546 | 200 µg/ml | $357.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 594 | sc-377002 AF594 | 200 µg/ml | $357.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 647 | sc-377002 AF647 | 200 µg/ml | $357.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 680 | sc-377002 AF680 | 200 µg/ml | $357.00 | |||
UBE2J1 Antibody (B-6) Alexa Fluor® 790 | sc-377002 AF790 | 200 µg/ml | $357.00 |