UBE2H Antibody (G-2): sc-515567
- UBE2H Antibody (G-2) is a mouse monoclonal IgG1 κ UBE2H antibody provided at 200 µg/ml
- raised against amino acids 1-183 representing full length UBE2H of human origin
- UBE2H Antibody (G-2) is recommended for detection of UBE2H of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-UBE2H Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE2H Antibody (G-2) for WB applications. These reagents are now offered in bundles with UBE2H Antibody (G-2) (see ordering information below).
UBE2H Antibody (G-2) is a mouse monoclonal IgG1 antibody that detects UBE2H protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBE2H (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE2H, also known as ubiquitin-conjugating enzyme E2H, UBC8, UBCH, UBCH2, or E2-20K, is a 183 amino acid protein that plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBE2H is primarily located in the cytoplasm and nucleus, where UBE2H facilitates the covalent attachment of ubiquitin to target proteins, a process vital for tagging proteins for degradation or altering their cellular location and function. UBE2H is particularly important for the degradation of histone H2A, thereby influencing gene expression and chromatin dynamics. The conservation of UBE2H across species, with 100% identity to its mouse counterpart and 98% identity to homologs in frogs and zebrafish, underscores its fundamental role in cellular processes. Multiple isoforms of UBE2H arise from alternative splicing, which may contribute to its functional diversity in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE2H Antibody (G-2) References:
- Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32. | Hayashida, S., et al. 2000. Genomics. 66: 221-5. PMID: 10860668
- TNF-alpha increases ubiquitin-conjugating activity in skeletal muscle by up-regulating UbcH2/E220k. | Li, YP., et al. 2003. FASEB J. 17: 1048-57. PMID: 12773487
- Mutation screening and association study of the UBE2H gene on chromosome 7q32 in autistic disorder. | Vourc'h, P., et al. 2003. Psychiatr Genet. 13: 221-5. PMID: 14639049
- Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. | Chuang, TH. and Ulevitch, RJ. 2004. Nat Immunol. 5: 495-502. PMID: 15107846
- Topors functions as an E3 ubiquitin ligase with specific E2 enzymes and ubiquitinates p53. | Rajendra, R., et al. 2004. J Biol Chem. 279: 36440-4. PMID: 15247280
- Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation. | Wefes, I., et al. 1995. Proc Natl Acad Sci U S A. 92: 4982-6. PMID: 7761435
- A human ubiquitin-conjugating enzyme homologous to yeast UBC8. | Kaiser, P., et al. 1994. J Biol Chem. 269: 8797-802. PMID: 8132613
- Characterization of functionally independent domains in the human ubiquitin conjugating enzyme UbcH2. | Kaiser, P., et al. 1995. FEBS Lett. 377: 193-6. PMID: 8543049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2H Antibody (G-2) | sc-515567 | 200 µg/ml | $316.00 | |||
UBE2H Antibody (G-2): m-IgG Fc BP-HRP Bundle | sc-531420 | 200 µg Ab; 10 µg BP | $354.00 | |||
UBE2H Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-525106 | 200 µg Ab, 40 µg BP | $354.00 | |||
UBE2H Antibody (G-2): m-IgG1 BP-HRP Bundle | sc-544544 | 200 µg Ab; 20 µg BP | $354.00 | |||
UBE2H Antibody (G-2) AC | sc-515567 AC | 500 µg/ml, 25% agarose | $416.00 | |||
UBE2H Antibody (G-2) HRP | sc-515567 HRP | 200 µg/ml | $316.00 | |||
UBE2H Antibody (G-2) FITC | sc-515567 FITC | 200 µg/ml | $330.00 | |||
UBE2H Antibody (G-2) PE | sc-515567 PE | 200 µg/ml | $343.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 488 | sc-515567 AF488 | 200 µg/ml | $357.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 546 | sc-515567 AF546 | 200 µg/ml | $357.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 594 | sc-515567 AF594 | 200 µg/ml | $357.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 647 | sc-515567 AF647 | 200 µg/ml | $357.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 680 | sc-515567 AF680 | 200 µg/ml | $357.00 | |||
UBE2H Antibody (G-2) Alexa Fluor® 790 | sc-515567 AF790 | 200 µg/ml | $357.00 |