UBE2G2 Antibody (D-4): sc-393780
- UBE2G2 Antibody (D-4) is a mouse monoclonal IgG1 κ UBE2G2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-64 mapping at the N-terminus of UBE2G2 of human origin
- UBE2G2 Antibody (D-4) is recommended for detection of UBE2G2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- Anti-UBE2G2 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE2G2 Antibody (D-4) for WB applications. These reagents are now offered in bundles with UBE2G2 Antibody (D-4) (see ordering information below).
UBE2G2 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBE2G2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-UBE2G2 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE2G2, also known as Ubiquitin-conjugating enzyme E2 G2 or UBC7, is a 165 amino acid protein that plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBE2G2 is primarily located in the cytoplasm and endoplasmic reticulum, where UBE2G2 facilitates the ubiquitination process by catalyzing the transfer of ubiquitin molecules to target proteins, marking them for degradation. UBE2G2′s cellular location is important for involvement in endoplasmic reticulum-associated degradation (ERAD), a pathway that eliminates misfolded or unassembled proteins, thereby preventing the accumulation of potentially toxic proteins within the cell. UBE2G2 shares 100% sequence identity with its mouse counterpart and exists in two isoforms due to alternative splicing, further highlighting UBE2G2′s significance in various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE2G2 Antibody (D-4) References:
- Inositol 1,4,5-trisphosphate receptor ubiquitination is mediated by mammalian Ubc7, a component of the endoplasmic reticulum-associated degradation pathway, and is inhibited by chelation of intracellular Zn2+. | Webster, JM., et al. 2003. J Biol Chem. 278: 38238-46. PMID: 12869571
- Endoplasmic reticulum-associated degradation of the human type 2 iodothyronine deiodinase (D2) is mediated via an association between mammalian UBC7 and the carboxyl region of D2. | Kim, BW., et al. 2003. Mol Endocrinol. 17: 2603-12. PMID: 12933904
- Expression, purification, and structural analysis of (HIS)UBE2G2 (human ubiquitin-conjugating enzyme). | Reyes, LF., et al. 2006. Protein Expr Purif. 45: 324-8. PMID: 16214370
- The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. | Chen, B., et al. 2006. Proc Natl Acad Sci U S A. 103: 341-6. PMID: 16407162
- Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7). | Arai, R., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 330-4. PMID: 16582478
- A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. | Li, W., et al. 2007. Nature. 446: 333-7. PMID: 17310145
- Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae ubc7 gene. | Katsanis, N. and Fisher, EM. 1998. Genomics. 51: 128-31. PMID: 9693041
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2G2 Antibody (D-4) | sc-393780 | 200 µg/ml | $322.00 | |||
UBE2G2 Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-530579 | 200 µg Ab; 10 µg BP | $361.00 | |||
UBE2G2 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-524087 | 200 µg Ab, 40 µg BP | $361.00 | |||
UBE2G2 Antibody (D-4): m-IgG1 BP-HRP Bundle | sc-544142 | 200 µg Ab; 20 µg BP | $361.00 | |||
UBE2G2 Antibody (D-4) AC | sc-393780 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UBE2G2 Antibody (D-4) HRP | sc-393780 HRP | 200 µg/ml | $322.00 | |||
UBE2G2 Antibody (D-4) FITC | sc-393780 FITC | 200 µg/ml | $336.00 | |||
UBE2G2 Antibody (D-4) PE | sc-393780 PE | 200 µg/ml | $349.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 488 | sc-393780 AF488 | 200 µg/ml | $364.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 546 | sc-393780 AF546 | 200 µg/ml | $364.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 594 | sc-393780 AF594 | 200 µg/ml | $364.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 647 | sc-393780 AF647 | 200 µg/ml | $364.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 680 | sc-393780 AF680 | 200 µg/ml | $364.00 | |||
UBE2G2 Antibody (D-4) Alexa Fluor® 790 | sc-393780 AF790 | 200 µg/ml | $364.00 |