UBE1 Antibody (D-8): sc-515424

UBE1 Antibody (D-8) is a mouse monoclonal IgG2a antibody that detects UBE1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBE1, or ubiquitin activating enzyme E1, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating protein degradation and maintaining cellular homeostasis. By catalyzing the first step in ubiquitin conjugation, UBE1 activates ubiquitin through an ATP-dependent process, forming a thiolester bond with a cysteine residue in UBE1 itself. This activated ubiquitin is then transferred to ubiquitin-conjugating enzymes, which subsequently attach ubiquitin to target substrates, marking them for degradation. The proper functioning of UBE1 is vital, as dysregulation of this pathway can lead to various diseases, including cancer and neurodegenerative disorders. Notably, the UBE1 gene is an example of an X-Y homologous gene, being X-linked with a distinct Y-linked counterpart in many mammals, although no homolog is found on the human Y chromosome. Interestingly, UBE1 is believed to escape X inactivation in humans, which may have implications for gene dosage and expression in females. Anti-UBE1 antibody (D-8) is an invaluable tool for researchers studying the dynamics of protein degradation and the intricate mechanisms of cellular regulation.

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UBE1 Antibody (D-8) References:

1. The ubiquitin-activating enzyme E1-like protein in lung cancer cell lines.  |  McLaughlin, PM., et al. 2000. Int J Cancer. 85: 871-6. PMID: 10709110
2. Ubiquitin-activating enzyme, E1, is associated with maturation of autophagic vacuoles.  |  Lenk, SE., et al. 1992. J Cell Biol. 118: 301-8. PMID: 1321157
3. Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.  |  Schwartz, AL., et al. 1992. Proc Natl Acad Sci U S A. 89: 5542-6. PMID: 1376922
4. The human ubiquitin-activating enzyme E1 gene (UBE1) mapped to band Xp11.3----p11.23 by fluorescence in situ hybridization.  |  Takahashi, E., et al. 1992. Cytogenet Cell Genet. 59: 268-9. PMID: 1544321
5. The ubiquitin-activating enzyme (E1) of the early-branching eukaryote Giardia intestinalis shows unusual proteolytic modifications and play important roles during encystation.  |  Niño, CA., et al. 2012. Acta Trop. 123: 39-46. PMID: 22498829
6. Comparative Proteomic Analysis of Wild-Type and SAP Domain Mutant Foot-and-Mouth Disease Virus-Infected Porcine Cells Identifies the Ubiquitin-Activating Enzyme UBE1 Required for Virus Replication.  |  Zhu, Z., et al. 2015. J Proteome Res. 14: 4194-206. PMID: 26354183
7. Ubiquitination-activating enzymes UBE1 and UBA6 regulate ubiquitination and expression of cardiac sodium channel Nav1.5.  |  Hu, Y., et al. 2020. Biochem J. 477: 1683-1700. PMID: 32315024
8. PYR-41, an inhibitor of ubiquitin-activating enzyme E1, attenuates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in mice.  |  Yang, L., et al. 2023. FASEB J. 37: e23210. PMID: 37738047
9. Ubiquitin-Activating Enzyme E1 (UBA1) as a Prognostic Biomarker and Therapeutic Target in Breast Cancer: Insights into Immune Infiltration and Functional Implications.  |  Feng, M., et al. 2024. Int J Mol Sci. 25: PMID: 39684409
10. Ubiquitin-activating enzyme, E1, is phosphorylated in mammalian cells by the protein kinase Cdc2.  |  Nagai, Y., et al. 1995. J Cell Sci. 108 (Pt 6): 2145-52. PMID: 7673335

The preferred UBE1 antibody is UBE1 Antibody (2G2): sc-53555.