UBE1 Antibody (2G2): sc-53555

UBE1 Antibody (2G2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the UBE1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-UBE1 antibody (2G2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ubiquitin activating enzyme E1 (UBE1) plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBE1 catalyzes the initial step of ubiquitin conjugation by adenylating the C-terminal glycine residue of ubiquitin in an ATP-dependent manner, forming a thiolester bond with a cysteine residue on UBE1. This activated ubiquitin is subsequently transferred to a ubiquitin-conjugating enzyme, which then donates the ubiquitin moiety to target substrates, marking them for degradation. Proper functioning of UBE1 is vital, as dysregulation of this pathway can lead to various diseases, including cancer and neurodegenerative disorders. Notably, the UBE1 gene is an example of an X-Y homologous gene, being X-linked with a distinct Y-linked counterpart in many mammals, although no UBE1 homolog is found on the human Y chromosome. UBE1 is believed to escape X inactivation in humans, which may have implications for gene dosage and expression in females.

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UBE1 Antibody (2G2) References:

1. Mapping and expression of the ubiquitin-activating enzyme E1 (Ube1) gene in the mouse.  |  Disteche, CM., et al. 1992. Mamm Genome. 3: 156-61. PMID: 1617221
2. Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.  |  Handley, PM., et al. 1991. Proc Natl Acad Sci U S A. 88: 258-62. PMID: 1986373
3. The ubiquitin E1 enzyme Ube1 mediates NEDD8 activation under diverse stress conditions.  |  Leidecker, O., et al. 2012. Cell Cycle. 11: 1142-50. PMID: 22370482
4. Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation.  |  Bialas, J., et al. 2015. PLoS One. 10: e0120329. PMID: 25768649
5. The ubiquitin-activating enzyme E1 as a novel therapeutic target for the treatment of restenosis.  |  Qin, Z., et al. 2016. Atherosclerosis. 247: 142-53. PMID: 26919560
6. Ubiquitin-activating enzyme E1 inhibitor PYR-41 retards sperm enlargement after fusion to the egg.  |  Yoshida, K., et al. 2018. Reprod Toxicol. 76: 71-77. PMID: 29355596
7. The pivotal role of ubiquitin-activating enzyme E1 (UBA1) in neuronal health and neurodegeneration.  |  Lambert-Smith, IA., et al. 2020. Int J Biochem Cell Biol. 123: 105746. PMID: 32315770
8. Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylation.  |  Oki, N., et al. 2022. Biochem Biophys Res Commun. 625: 94-101. PMID: 35952613
9. A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status.  |  Coleman, MP., et al. 1996. Genomics. 31: 135-8. PMID: 8808293
10. The origin and loss of the ubiquitin activating enzyme gene on the mammalian Y chromosome.  |  Mitchell, MJ., et al. 1998. Hum Mol Genet. 7: 429-34. PMID: 9467000
11. X inactivation analysis and DNA methylation studies of the ubiquitin activating enzyme E1 and PCTAIRE-1 genes in human and mouse.  |  Carrel, L., et al. 1996. Hum Mol Genet. 5: 391-401. PMID: 8852665
12. Transcriptional analysis of the candidate spermatogenesis gene Ube1y and of the closely related Ube1x shows that they are coexpressed in spermatogonia and spermatids but are repressed in pachytene spermatocytes.  |  Odorisio, T., et al. 1996. Dev Biol. 180: 336-43. PMID: 8948595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UBE1 Antibody (2G2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-UBE1 Antibody (2G2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UBE1 Antibody (2G2) Specifications

Alternate Product Name: UBE1

Product Clone Name: 2G2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: UBE1

Epitope Species: human

Product Reactivity/Detection: UBE1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for UBE1 Antibody (2G2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin