Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UBC12 CRISPR/Cas9 KO Plasmid (m): sc-423575
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBC12 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBC12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UBC12 Antibody (D-4): sc-390064
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBC12 CRISPR/Cas9 KO Plasmid (m) | sc-423575 | 20 µg | $397.00 |
Overview
Ube2m encodes UBC12, an E2 conjugating enzyme that transfers NEDD8 to cullin scaffolds, enabling activation of cullin–RING ubiquitin ligases (CRLs) and broad control of proteostasis. Through regulation of CRL-dependent ubiquitination, UBC12 impacts cell-cycle progression, DNA damage responses, signal transduction, and stress adaptation by modulating turnover of key regulatory proteins. Perturbation of neddylation can disrupt protein homeostasis and checkpoint control, linking Ube2m/UBC12 function to pathways frequently interrogated in cancer biology, neurobiology, and inflammatory signaling. In mouse systems, Ube2m loss-of-function studies support mechanistic dissection of ubiquitin-like modification networks and their contributions to tissue development and disease-relevant phenotypes.
UBC12 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ube2m gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ube2m together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ube2m open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBC12 protein expression.
This CRISPR knockout system enables efficient generation of Ube2m-deficient cell models for investigation of UBC12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ube2m exon(s) critical for UBC12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ube2m genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBC12 CRISPR/Cas9 KO Plasmid (m) and UBC12 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ube2m locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBC12 HDR Plasmid (m) and UBC12 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ube2m homology arms to support homology-directed repair at defined Ube2m target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.