UBC12 Antibody (D-4): sc-390064
- UBC12 Antibody (D-4) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 1-42 mapping at the N-terminus of UBC12 of human origin
- Anti-UBC12 Antibody (D-4) is recommended for detection of UBC12 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
- Anti-UBC12 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for UBC12 Antibody (D-4) for WB applications. This reagent is now offered in a bundle with UBC12 Antibody (D-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
UBC12 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBC12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBC12 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBC12, also known as UBE2M, is a 183 amino acid member of the E2 ubiquitin-conjugating enzyme family, which plays a crucial role in the ubiquitination process—a vital mechanism for regulating protein degradation and cellular homeostasis. UBC12 is particularly important because UBC12 is involved in the conjugation of NEDD8, a ubiquitin-like protein, to target proteins, thereby influencing various cellular processes, including cell cycle progression and signal transduction. The ability of UBC12 to facilitate the attachment of NEDD8 is essential for the regulation of protein stability and function, making UBC12 a key player in cellular proliferation and response to stress. Using anti-UBC12 antibody (D-4) in research can provide valuable insights into the mechanisms of protein regulation and the implications of ubiquitination in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBC12 Antibody (D-4) References:
- Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. | Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
- A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo. | Wada, H., et al. 2000. J Biol Chem. 275: 17008-15. PMID: 10828074
- A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. | Huang, DT., et al. 2004. Nat Struct Mol Biol. 11: 927-35. PMID: 15361859
- Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. | Huang, DT., et al. 2005. Mol Cell. 17: 341-50. PMID: 15694336
- Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. | Huang, DT., et al. 2007. Nature. 445: 394-8. PMID: 17220875
- Ubiquitin, proteasomes, and the regulation of intracellular protein degradation. | Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
- The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins. | Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
- A new NEDD8-ligating system for cullin-4A. | Osaka, F., et al. 1998. Genes Dev. 12: 2263-8. PMID: 9694792
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBC12 Antibody (D-4) | sc-390064 | 200 µg/ml | $316.00 | |||
UBC12 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-523392 | 200 µg Ab, 40 µg BP | $354.00 | |||
UBC12 Antibody (D-4) AC | sc-390064 AC | 500 µg/ml, 25% agarose | $416.00 | |||
UBC12 Antibody (D-4) HRP | sc-390064 HRP | 200 µg/ml | $316.00 | |||
UBC12 Antibody (D-4) FITC | sc-390064 FITC | 200 µg/ml | $330.00 | |||
UBC12 Antibody (D-4) PE | sc-390064 PE | 200 µg/ml | $343.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 488 | sc-390064 AF488 | 200 µg/ml | $357.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 546 | sc-390064 AF546 | 200 µg/ml | $357.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 594 | sc-390064 AF594 | 200 µg/ml | $357.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 647 | sc-390064 AF647 | 200 µg/ml | $357.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 680 | sc-390064 AF680 | 200 µg/ml | $357.00 | |||
UBC12 Antibody (D-4) Alexa Fluor® 790 | sc-390064 AF790 | 200 µg/ml | $357.00 |