Date published: 2026-4-5

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UBA3 Antibody (F-10): sc-377212

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Datasheets
  • UBA3 Antibody (F-10) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 317-463 mapping at the C-terminus of UBA3 of human origin
  • recommended for detection of UBA3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for UBA3 Antibody (F-10) for WB and IHC(P) applications. These reagents are now offered in bundles with UBA3 Antibody (F-10) (see ordering information below).
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UBA3 Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects UBA3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). UBA3, also known as the NEDD8-activating enzyme E1 catalytic subunit or UBE1C, is a 463 amino acid protein that plays a crucial role in the ubiquitin-like modification pathway, specifically in the activation of NEDD8, a protein that is vital for regulating numerous cellular processes such as cell division, signaling, and embryogenesis. Anti-UBA3 antibody (F-10) facilitates the conjugation of NEDD8 to target proteins, which is essential for the proper functioning of various cellular mechanisms, including the regulation of protein stability and activity. UBA3 is ubiquitously expressed and exists in two isoforms due to alternative splicing, highlighting its versatile role in cellular functions. UBA3 (F-10) antibody is an invaluable tool for researchers aiming to explore the dynamics of NEDD8 modification and its implications in cellular processes and disease states.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

UBA3 Antibody (F-10)

sc-377212
200 µg/ml
$322.00

UBA3 Antibody (F-10): m-IgG Fc BP-HRP Bundle

sc-540703
200 µg Ab; 10 µg BP
$361.00

UBA3 Antibody (F-10): m-IgG1 BP-HRP Bundle

sc-542311
200 µg Ab; 20 µg BP
$361.00

Can UBA3 (F-10): sc-377212 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, UBA3 (F-10): sc-377212 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced superb immunoperoxidase nuclearProduced superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-02-14
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UBA3 Antibody (F-10) is rated 5.0 out of 5 by 2.
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