U2AF65 Antibody (D-8): sc-374333
- U2AF65 Antibody (D-8) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-25 at the N-terminus of U2AF65 of human origin
- recommended for detection of U2AF65 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- m-IgGκ BP-HRP is the preferred secondary detection reagent for U2AF65 Antibody (D-8) for WB applications. This reagent is now offered in a bundle with U2AF65 Antibody (D-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
U2AF65 Antibody (D-8) is a mouse monoclonal IgM antibody that detects U2AF65 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). U2AF65 is a critical component of the U2 auxiliary factor (U2AF) complex, which plays a vital role in pre-mRNA splicing by recognizing the branch point sequence and recruiting U2 small nuclear ribonucleoprotein (snRNP) to the spliceosome. U2AF65 is predominantly located in the nucleus, where U2AF65 facilitates spliceosome assembly, a complex necessary for intron removal from pre-mRNA. Proper localization of U2AF65 ensures spliceosome components are correctly positioned to execute splicing reactions. Disruption of U2AF65 function or localization can lead to aberrant splicing, implicated in various diseases, including cancer and neurodegenerative disorders. U2AF65 interacts with other spliceosomal proteins, including U2AF35, forming a heterodimer crucial for splicing. U2AF complex′s ability to recognize specific RNA sequences highlights U2AF65′s importance in gene expression regulation and cellular homeostasis maintenance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
U2AF65 Antibody (D-8) References:
- Fas splicing regulation during early apoptosis is linked to caspase-mediated cleavage of U2AF65. | Izquierdo, JM. 2008. Mol Biol Cell. 19: 3299-307. PMID: 18508922
- Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65. | Jenkins, JL., et al. 2008. J Biol Chem. 283: 33641-9. PMID: 18842594
- The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. | David, CJ., et al. 2011. Genes Dev. 25: 972-83. PMID: 21536736
- Binding of hnRNP H and U2AF65 to respective G-codes and a poly-uridine tract collaborate in the N50-5'ss selection of the REST N exon in H69 cells. | Ortuño-Pineda, C., et al. 2012. PLoS One. 7: e40315. PMID: 22792276
- Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 3'-splice site recognition. | Zhang, Y., et al. 2013. Nucleic Acids Res. 41: 1343-54. PMID: 23175611
- Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. | Chen, L., et al. 2017. Nucleic Acids Res. 45: 2051-2067. PMID: 27683217
- Differential regulation of sFlt-1 splicing by U2AF65 and JMJD6 in placental-derived and endothelial cells. | Eddy, AC., et al. 2020. Biosci Rep. 40: PMID: 32039444
- Cancer-Associated Substitutions in RNA Recognition Motifs of PUF60 and U2AF65 Reveal Residues Required for Correct Folding and 3' Splice-Site Selection. | Kralovicova, J., et al. 2020. Cancers (Basel). 12: PMID: 32664474
- Biochemical properties of a novel U2AF65 protein isoform generated by alternative RNA splicing. | Ding, F., et al. 1996. Biochem Biophys Res Commun. 224: 675-83. PMID: 8713106
- U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction. | Fleckner, J., et al. 1997. Genes Dev. 11: 1864-72. PMID: 9242493
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
U2AF65 Antibody (D-8) | sc-374333 | 200 µg/ml | $322.00 | |||
U2AF65 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-550748 | 200 µg Ab; 40 µg BP | $361.00 | |||
U2AF65 (D-8) Neutralizing Peptide | sc-374333 P | 100 µg/0.5 ml | $69.00 |