U1 snRNP 70 Antibody (C-3): sc-390899

U1 snRNP 70 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects U1 snRNP 70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-U1 snRNP 70 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. U1 snRNP 70 plays a critical role in the RNA spliceosome, a complex essential for accurate removal of introns from pre-messenger RNA, thereby facilitating production of mature mRNA necessary for protein synthesis. U1 snRNP 70 specifically binds to the single-stranded loop of hairpin 1 on U1 small nuclear RNA, and RNA binding domain, consisting of 80-90 amino acids, is crucial for this interaction, which occurs independently of ATP. U1 snRNP 70 binding to U1 snRNA is vital for spliceosome assembly and recruitment of additional splicing factors, ensuring fidelity of gene expression. U1 snRNP 70 is dynamically localized within cells, initially dispersing throughout cytoplasm during early stages of mitosis and subsequently accumulating in daughter nuclei as mitosis progresses, highlighting importance in cellular division and RNA processing.

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U1 snRNP 70 Antibody (C-3) References:

1. Distribution and antigen specificity of anti-U1RNP antibodies in patients with systemic sclerosis.  |  Ihn, H., et al. 1999. Clin Exp Immunol. 117: 383-7. PMID: 10444274
2. The appearance of U1 RNP antibody specificities in sequential autoimmune human antisera follows a characteristic order that implicates the U1-70 kd and B'/B proteins as predominant U1 RNP immunogens.  |  Greidinger, EL. and Hoffman, RW. 2001. Arthritis Rheum. 44: 368-75. PMID: 11229468
3. Apoptotic U1-70 kd is antigenically distinct from the intact form of the U1-70-kd molecule.  |  Greidinger, EL., et al. 2002. Arthritis Rheum. 46: 1264-9. PMID: 12115232
4. A new conformational epitope generated by the binding of recombinant 70-kd protein and U1 RNA to anti-U1 RNP autoantibodies in sera from patients with mixed connective tissue disease.  |  Murakami, A., et al. 2002. Arthritis Rheum. 46: 3273-82. PMID: 12483732
5. Human parvovirus B19 nonstructural protein NS1 enhanced the expression of cleavage of 70 kDa U1-snRNP autoantigen.  |  Tzang, BS., et al. 2010. J Biomed Sci. 17: 40. PMID: 20500824
6. Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.  |  Surowy, CS., et al. 1989. Mol Cell Biol. 9: 4179-86. PMID: 2531275
7. A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.  |  Query, CC., et al. 1989. Mol Cell Biol. 9: 4872-81. PMID: 2532301
8. In vitro assembly of U1 snRNPs.  |  Hamm, J., et al. 1987. EMBO J. 6: 3479-85. PMID: 2962858
9. Clinical significance of small nuclear ribonucleoprotein U1 subunit 70 in patients with hepatocellular carcinoma.  |  Jiang, D., et al. 2024. PeerJ. 12: e16876. PMID: 38500533
10. Deciphering the Reactivity of Autoantibodies Directed against the RNP-A, -C and 70 kDa Components of the U1-snRNP Complex: 'Double or Nothing'?  |  Bertin, D., et al. 2024. Biomedicines. 12: PMID: 39062124
11. U1 small nuclear ribonucleoprotein studied by in vitro assembly.  |  Wieben, ED., et al. 1983. J Cell Biol. 96: 1751-5. PMID: 6133878
12. Differential interaction of splicing snRNPs with coiled bodies and interchromatin granules during mitosis and assembly of daughter cell nuclei.  |  Ferreira, JA., et al. 1994. J Cell Biol. 126: 11-23. PMID: 8027171

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-U1 snRNP 70 Antibody (C-3) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-U1 snRNP 70 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

U1 snRNP 70 Antibody (C-3) Specifications

Alternate Product Name: SNRNP70

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 183-280

Epitope Target: U1 snRNP 70

Epitope Species: human

Product Reactivity/Detection: U1 snRNP 70

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for U1 snRNP 70 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred U1 snRNP 70 antibody is U1 snRNP 70 Antibody (E-4): sc-390988.