Tyk 2 Antibody (C-8) recommended for detection of TYk 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Tyk 2 Antibody (C-8): sc-5271

Tyk 2 Antibody (C-8) is rated 4.3 out of 5 by 18.
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Datasheets

    • Anti-Tyk 2 Antibody (C-8) is a mouse monoclonal IgG1 (kappa light chain) Tyk 2 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1170-1187 at the C-terminus of Tyk 2 of human origin
    • Anti-Tyk 2 Antibody (C-8) is recommended for detection of TYk 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Tyk 2 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-5271 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tyk 2 (C-8): sc-5271.
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I tried to detect tyk2 band from mouse brain lysate in IB (using LI-COR) and couldn't detect any thing. I loaded 20 ug of brain lysate. What condition do I need to use to detect tyk2 band? Thanks.

Asked by: jiyoen kim
Thank you for your question. We have not tested this antibody in brain tissue extracts. For the Western blot data on our website, we load 50 ug whole cell lysate protein. The primary antibody was used at a dilution of 1:200. Please refer to our Western blotting protocol for additional details. And contact us for further Technical Support with using this antibody.
Answered by: Technical Support
Date published: 2019-05-25

I am using Tyk 2 (C-8): sc-5271 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-02
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Rated 4 out of 5 by from works great for IP, not verified in knockout cells I used this at 1:50 in lysates from a human T cell line for immunoprecipitation. I also used it to blot for Tyk2 in whole cell lysates and the IP samples. It worked well and yielded a single band. The only caveat is that I don't have a Tyk2 deficient control to verify that it is recognizing Tyk2.
Date published: 2018-07-22
Rated 5 out of 5 by from Excellent result In the experiment,Tyk 2 antibody stains very brightly in rat, the staining intensity between these cells was obvious, it is highly suitable to show cellular staining.
Date published: 2017-10-09
Rated 5 out of 5 by from very good for IF It works extremely well with no background and clear nuclear labeling of positive cells.Concentration was use 1:250.
Date published: 2017-08-12
Rated 5 out of 5 by from good I can detect endogenous Tyk2 in 293 cell lysates and Tyk2 transfected 293 cell lysates.
Date published: 2017-08-04
Rated 4 out of 5 by from Superb immunoperoxidase cytoplasmic and membrane Superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue.
Date published: 2017-05-23
Rated 5 out of 5 by from WB This antibody is good for WB and the concentration I used is 1:500.
Date published: 2017-04-23
Rated 3 out of 5 by from 检测效果有点弱 有图有真相,在巨噬细胞里做的,条带位置大小是对的,用超敏发光液曝光的,真的很弱,可能巨噬细胞本身表达就很低
Date published: 2017-04-16
Rated 5 out of 5 by from specific result we're very happy with Tyk 2 Antibody. the antibody gave clear and specific results.
Date published: 2017-04-03
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