TXNDC4 Antibody (H-11): sc-515435

TXNDC4 Antibody (H-11) is a mouse monoclonal IgG1 antibody that detects TXNDC4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TXNDC4, also known as ERP44, is a 406 amino acid protein characterized by a single thioredoxin domain, which plays a crucial role in various redox reactions within the cell. TXNDC4 is primarily localized in the lumen of the endoplasmic reticulum (ER), where TXNDC4 is essential for maintaining calcium homeostasis by inhibiting the activity of inositol 1,4,5-triphosphate receptor type 1 (IP3R-I) within calcium channels. Additionally, TXNDC4 is involved in the regulation of oxidative protein folding and the retention of key proteins such as Ero1-Lβ and Ero1-Lα in the ER, which are vital for proper protein maturation and function. TXNDC4 expression increases in response to ER stress, highlighting TXNDC4′s significant role in preserving intraluminal conditions and ensuring cellular homeostasis. TXNDC4 features an N-terminal ER targeting sequence and a C-terminal ER retention signal (RDEL), both of which are critical for localization and function within the ER. Anti-TXNDC4 antibody (H-11) serves as an invaluable tool for researchers studying the intricate mechanisms of protein folding and calcium signaling in various biological contexts.

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TXNDC4 Antibody (H-11) References:

1. ERp44 mediates a thiol-independent retention of formylglycine-generating enzyme in the endoplasmic reticulum.  |  Mariappan, M., et al. 2008. J Biol Chem. 283: 6375-83. PMID: 18178549
2. ERp44 C160S/C212S mutants regulate IP3R1 channel activity.  |  Pan, C., et al. 2011. Protein Cell. 2: 990-6. PMID: 22183808
3. The role of ERp44 in maturation of serotonin transporter protein.  |  Freyaldenhoven, S., et al. 2012. J Biol Chem. 287: 17801-17811. PMID: 22451649
4. Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.  |  Wang, DY., et al. 2014. J Am Heart Assoc. 3: e001018. PMID: 25332179
5. Proteostasis and 'redoxtasis' in the secretory pathway: Tales of tails from ERp44 and immunoglobulins.  |  Anelli, T., et al. 2015. Free Radic Biol Med. 83: 323-30. PMID: 25744412
6. ERP44 inhibits human lung cancer cell migration mainly via IP3R2.  |  Huang, X., et al. 2016. Aging (Albany NY). 8: 1276-86. PMID: 27347718
7. Withdrawal: The role of ERp44 in maturation of serotonin transporter protein.  |  Freyaldenhoven, S., et al. 2019. J Biol Chem. 294: 70. PMID: 30610126
8. A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment.  |  Tempio, T., et al. 2021. iScience. 24: 102244. PMID: 33763635
9. Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia.  |  Yang, Y., et al. 2021. Bioengineered. 12: 9561-9574. PMID: 34719307
10. Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface.  |  Amagai, Y., et al. 2023. Nat Commun. 14: 2683. PMID: 37160917

The preferred TXNDC4 antibody is TXNDC4 Antibody (E-6): sc-393687.