TXNDC4 Antibody (E-6): sc-393687

TXNDC4 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TXNDC4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TXNDC4 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TXNDC4 protein, also known as ERP44, is a 406 amino acid protein characterized by a thioredoxin domain that plays a crucial role in redox reactions within the cell. Located in the lumen of the endoplasmic reticulum, TXNDC4 is essential for inhibiting the activity of inositol 1,4,5-triphosphate receptor type 1 (IP3R-I) in calcium channels, thereby regulating calcium signaling, which is vital for various cellular processes including muscle contraction, neurotransmitter release, and cell proliferation. Furthermore, TXNDC4 is implicated in oxidative protein folding and the retention of proteins such as Ero1-Lβ and Ero1-Lα within the endoplasmic reticulum, highlighting its importance in maintaining protein homeostasis under stress conditions. Expression of TXNDC4 is upregulated during endoplasmic reticulum stress, indicating a significant role in preserving the intraluminal environment necessary for proper protein maturation and function. Additionally, TXNDC4 features an N-terminal endoplasmic reticulum targeting sequence and a C-terminal endoplasmic reticulum retention signal (RDEL), which are critical for localization and function within the endoplasmic reticulum.

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TXNDC4 Antibody (E-6) References:

1. ERp44 mediates a thiol-independent retention of formylglycine-generating enzyme in the endoplasmic reticulum.  |  Mariappan, M., et al. 2008. J Biol Chem. 283: 6375-83. PMID: 18178549
2. From antibodies to adiponectin: role of ERp44 in sizing and timing protein secretion.  |  Cortini, M. and Sitia, R. 2010. Diabetes Obes Metab. 12 Suppl 2: 39-47. PMID: 21029299
3. ERp44 C160S/C212S mutants regulate IP3R1 channel activity.  |  Pan, C., et al. 2011. Protein Cell. 2: 990-6. PMID: 22183808
4. Segmental expression and C-terminal labeling of protein ERp44 through protein trans-splicing.  |  Dai, X., et al. 2015. Protein Expr Purif. 112: 29-36. PMID: 25907381
5. ERp44 Exerts Redox-Dependent Control of Blood Pressure at the ER.  |  Hisatsune, C., et al. 2015. Mol Cell. 58: 1015-27. PMID: 25959394
6. Knockdown of ERp44 leads to apoptosis via activation of ER stress in HeLa cells.  |  Chang, Y., et al. 2015. Biochem Biophys Res Commun. 463: 606-11. PMID: 26043695
7. ERP44 inhibits human lung cancer cell migration mainly via IP3R2.  |  Huang, X., et al. 2016. Aging (Albany NY). 8: 1276-86. PMID: 27347718
8. Adiponectin, a Therapeutic Target for Obesity, Diabetes, and Endothelial Dysfunction.  |  Achari, AE. and Jain, SK. 2017. Int J Mol Sci. 18: PMID: 28635626
9. Zinc regulates ERp44-dependent protein quality control in the early secretory pathway.  |  Watanabe, S., et al. 2019. Nat Commun. 10: 603. PMID: 30723194
10. A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment.  |  Tempio, T., et al. 2021. iScience. 24: 102244. PMID: 33763635
11. The differential placental expression of ERp44 and pre-eclampsia; association with placental zinc, the ERAP1 and the renin-angiotensin-system.  |  Raghu, R., et al. 2023. Placenta. 134: 9-14. PMID: 36848863
12. Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface.  |  Amagai, Y., et al. 2023. Nat Commun. 14: 2683. PMID: 37160917

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TXNDC4 Antibody (E-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TXNDC4 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TXNDC4 Antibody (E-6) Specifications

Alternate Product Name: TXNDC4

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 107-406

Epitope Target: TXNDC4

Epitope Species: human

Product Reactivity/Detection: TXNDC4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TXNDC4 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin