TXNDC4 Antibody (E-6) recommended for detection of TXNDC4 of mouse, rat and human origin by WB, IP, IF and ELISA

TXNDC4 Antibody (E-6): sc-393687

TXNDC4 Antibody (E-6) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-TXNDC4 Antibody (E-6) is a mouse monoclonal IgG1 TXNDC4 antibody provided at 200 µg/ml
    • raised against amino acids 107-406 mapping at the C-terminus of TXNDC4 of human origin
    • Anti-TXNDC4 Antibody (E-6) is recommended for detection of TXNDC4 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-TXNDC4 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TXNDC4 (E-6): sc-393687.
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While the staining shown here appears quite dispersed throughout the cytoplasm, I have found my staining of TXNDC4 to be somewhat localised to what looks like the Golgi (as well as general cytoplasm). Has this been seen before? Could it be the dilution?

Asked by: ladedadi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended fixation method for immunofluorescence staining with TXNDC4 (E-6): sc-393687 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from test concluant Bon marquage en immunofluorescence sur cellule mcf7 fixées PFA
Date published: 2018-01-10
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Great Western Blot data of TXNDC4 expression in K Great Western Blot data of TXNDC4 expression in K-562, Raji and A-431 whole cell lysates and human placenta tissue extract . -SCBT QC
Date published: 2013-06-30
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