Twinfilin-2 Antibody (B-6): sc-398673
- Twinfilin-2 Antibody (B-6) is a mouse monoclonal IgG2b κ Twinfilin-2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 36-61 within an internal region of Twinfilin-2 of human origin
- Twinfilin-2 Antibody (B-6) is recommended for detection of Twinfilin-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Twinfilin-2 Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Twinfilin-2 Antibody (B-6) for WB applications. These reagents are now offered in bundles with Twinfilin-2 Antibody (B-6) (see ordering information below).
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Twinfilin-2 Antibody (B-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Twinfilin-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Twinfilin-2 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Twinfilin-2, also known as TWF2, A6r, A6RP, or PTK9L, is a 349 amino acid protein that plays a crucial role in regulating actin dynamics within cells. Twinfilin-2 is primarily localized to the perinuclear region of the cytoplasm and cytoskeleton, where Twinfilin-2 contains two actin-depolymerizing factor homology (ADF-H) domains. This localization allows Twinfilin-2 to effectively bind and sequester G-actin, capping the barbed ends of actin filaments and inhibiting actin polymerization. These functions are vital for various actin-related processes, including cell motility and morphological changes, which are essential for normal cellular function and response to environmental cues. Twinfilin-2 undergoes post-translational modifications, such as phosphorylation by protein kinase C zeta, which can further modulate activity and interactions. The gene encoding Twinfilin-2 is located on human chromosome 3, a region encompassing over 1,100 genes, including those associated with chemokine receptors and various cancer-related loci, highlighting potential implications in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Twinfilin-2 Antibody (B-6) References:
- Cloning, expression and characterization of an A6-related protein. | Rohwer, A., et al. 1999. Eur J Biochem. 263: 518-25. PMID: 10406962
- Twinfilin, a molecular mailman for actin monomers. | Palmgren, S., et al. 2002. J Cell Sci. 115: 881-6. PMID: 11870207
- Mammals have two twinfilin isoforms whose subcellular localizations and tissue distributions are differentially regulated. | Vartiainen, MK., et al. 2003. J Biol Chem. 278: 34347-55. PMID: 12807912
- Identification of twinfilin-2 as a factor involved in neurite outgrowth by RNAi-based screen. | Yamada, S., et al. 2007. Biochem Biophys Res Commun. 363: 926-30. PMID: 17910947
- Two biochemically distinct and tissue-specific twinfilin isoforms are generated from the mouse Twf2 gene by alternative promoter usage. | Nevalainen, EM., et al. 2009. Biochem J. 417: 593-600. PMID: 18837697
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Twinfilin-2 Antibody (B-6) | sc-398673 | 200 µg/ml | $316.00 | |||
Twinfilin-2 Antibody (B-6): m-IgGκ BP-HRP Bundle | sc-524449 | 200 µg Ab, 40 µg BP | $354.00 | |||
Twinfilin-2 Antibody (B-6): m-IgG2b BP-HRP Bundle | sc-549161 | 200 µg Ab; 10 µg BP | $354.00 | |||
Twinfilin-2 Antibody (B-6) AC | sc-398673 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Twinfilin-2 Antibody (B-6) HRP | sc-398673 HRP | 200 µg/ml | $316.00 | |||
Twinfilin-2 Antibody (B-6) FITC | sc-398673 FITC | 200 µg/ml | $330.00 | |||
Twinfilin-2 Antibody (B-6) PE | sc-398673 PE | 200 µg/ml | $343.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 488 | sc-398673 AF488 | 200 µg/ml | $357.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 546 | sc-398673 AF546 | 200 µg/ml | $357.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 594 | sc-398673 AF594 | 200 µg/ml | $357.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 647 | sc-398673 AF647 | 200 µg/ml | $357.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 680 | sc-398673 AF680 | 200 µg/ml | $357.00 | |||
Twinfilin-2 Antibody (B-6) Alexa Fluor® 790 | sc-398673 AF790 | 200 µg/ml | $357.00 | |||
Twinfilin-2 (B-6) Neutralizing Peptide | sc-398673 P | 100 µg/0.5 ml | $68.00 |