Tryptase ε Antibody (G-9): sc-377427
- Tryptase ε Antibody (G-9) is a mouse monoclonal IgG2b κ Tryptase ε antibody provided at 200 µg/ml
- raised against amino acids 108-160 mapping within an internal region of Tryptase ε of human origin
- Tryptase epsilon Antibody (G-9) is recommended for detection of Tryptase ε of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Tryptase epsilon Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Tryptase ε Antibody (G-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Tryptase ε Antibody (G-9) (see ordering information below).
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Tryptase ε Antibody (G-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects Tryptase epsilon protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Tryptase ε antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tryptase ε, also known as brain-specific serine protease 4 (BSSP-4) or serine protease 22, is a member of the human 16p13.3 family of serine proteases and is primarily expressed in the pulmonary epithelium, where Tryptase ε plays a crucial role in respiratory health. Tryptase ε is secreted as an active enzyme and is unique among its family members due to an ability to autoactivate, which means that once activated, Tryptase ε cannot be effectively inhibited by protease inhibitors present in normal plasma. Tryptase ε is a potent activator of urokinase-type plasminogen activator precursor (uPA), a serine protease essential for converting plasminogen into its active form, thereby facilitating fibrinolysis, connective tissue remodeling, and innate immune responses. The regulation of Tryptase ε is vital for maintaining homeostasis in the lung environment, and dysregulation can lead to pathological conditions, making Tryptase ε (G-9) antibody an important tool for research in respiratory diseases and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tryptase ε Antibody (G-9) References:
- Human tryptase epsilon (PRSS22), a new member of the chromosome 16p13.3 family of human serine proteases expressed in airway epithelial cells. | Wong, GW., et al. 2001. J Biol Chem. 276: 49169-82. PMID: 11602603
- Membrane anchored serine proteases: a rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer. | Netzel-Arnett, S., et al. 2003. Cancer Metastasis Rev. 22: 237-58. PMID: 12784999
- Mouse prostasin gene structure, promoter analysis, and restricted expression in lung and kidney. | Verghese, GM., et al. 2004. Am J Respir Cell Mol Biol. 30: 519-29. PMID: 12959947
- Mouse chromosome 17A3.3 contains 13 genes that encode functional tryptic-like serine proteases with distinct tissue and cell expression patterns. | Wong, GW., et al. 2004. J Biol Chem. 279: 2438-52. PMID: 14583634
- Urokinase-type plasminogen activator is a preferred substrate of the human epithelium serine protease tryptase epsilon/PRSS22. | Yasuda, S., et al. 2005. Blood. 105: 3893-901. PMID: 15701722
- Identification of a subgroup of glycosylphosphatidylinositol-anchored tryptases. | Wong, GW. and Stevens, RL. 2005. Biochem Biophys Res Commun. 336: 579-84. PMID: 16143303
- Expression and characterization of recombinant gamma-tryptase. | Yuan, J., et al. 2006. Protein Expr Purif. 49: 47-54. PMID: 16815034
- Gene expression profiling following constitutive activation of MEK1 and transformation of rat intestinal epithelial cells. | Komatsu, K., et al. 2006. Mol Cancer. 5: 63. PMID: 17112382
- The human urokinase-plasminogen activator gene and its promoter. | Riccio, A., et al. 1985. Nucleic Acids Res. 13: 2759-71. PMID: 2987867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tryptase ε Antibody (G-9) | sc-377427 | 200 µg/ml | $322.00 | |||
Tryptase ε Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-523326 | 200 µg Ab, 40 µg BP | $361.00 | |||
Tryptase ε Antibody (G-9): m-IgG2b BP-HRP Bundle | sc-549106 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tryptase ε Antibody (G-9) AC | sc-377427 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tryptase ε Antibody (G-9) HRP | sc-377427 HRP | 200 µg/ml | $322.00 | |||
Tryptase ε Antibody (G-9) FITC | sc-377427 FITC | 200 µg/ml | $336.00 | |||
Tryptase ε Antibody (G-9) PE | sc-377427 PE | 200 µg/ml | $349.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 488 | sc-377427 AF488 | 200 µg/ml | $364.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 546 | sc-377427 AF546 | 200 µg/ml | $364.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 594 | sc-377427 AF594 | 200 µg/ml | $364.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 647 | sc-377427 AF647 | 200 µg/ml | $364.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 680 | sc-377427 AF680 | 200 µg/ml | $364.00 | |||
Tryptase ε Antibody (G-9) Alexa Fluor® 790 | sc-377427 AF790 | 200 µg/ml | $364.00 |