Trypsin Antibody (D-1): sc-137077

Trypsin Antibody (D-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Trypsin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Trypsin (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Trypsin plays a crucial role in the digestive system by cleaving peptide bonds in proteins, thereby activating other digestive enzymes and facilitating the breakdown of dietary proteins into absorbable amino acids. This enzymatic activity is vital for proper nutrient absorption and overall digestive health. The human pancreas secretes three different isoforms of inactive trypsinogen into the small intestine, which are converted into active forms by Enterokinase. Mutations in the gene encoding Trypsin-1 that enhance activity are linked to autosomal dominant hereditary pancreatitis, a condition causing severe abdominal pain and other complications. Trypsin-3 is uniquely expressed in the brain, indicating potential roles beyond digestion, which may have implications for neurological health.

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Trypsin Antibody (D-1) References:

1. Chronic pancreatitis: pathogenesis and molecular aspects.  |  Kleeff, J., et al. 2000. Ann Ital Chir. 71: 3-10. PMID: 10829517
2. Absence of PRSS1 mutations and association of SPINK1 trypsin inhibitor mutations in hereditary and non-hereditary chronic pancreatitis.  |  Chandak, GR., et al. 2004. Gut. 53: 723-8. PMID: 15082592
3. Chymotrypsin C (caldecrin) stimulates autoactivation of human cationic trypsinogen.  |  Nemoda, Z. and Sahin-Tóth, M. 2006. J Biol Chem. 281: 11879-86. PMID: 16505482
4. The role of trypsin, trypsin inhibitor, and trypsin receptor in the onset and aggravation of pancreatitis.  |  Hirota, M., et al. 2006. J Gastroenterol. 41: 832-6. PMID: 17048046
5. Human cationic trypsinogen is sulfated on Tyr154.  |  Sahin-Tóth, M., et al. 2006. FEBS J. 273: 5044-50. PMID: 17087724
6. Association of cystic fibrosis transmembrane conductance regulator (CFTR) mutation/variant/haplotype and tumor necrosis factor (TNF) promoter polymorphism in hyperlipidemic pancreatitis.  |  Chang, YT., et al. 2008. Clin Chem. 54: 131-8. PMID: 17981921
7. Hereditary chronic pancreatitis.  |  Teich, N. and Mössner, J. 2008. Best Pract Res Clin Gastroenterol. 22: 115-30. PMID: 18206817
8. Role of genetic disorders in acute recurrent pancreatitis.  |  Keim, V. 2008. World J Gastroenterol. 14: 1011-5. PMID: 18286680
9. Influence of Asp Isomerization on Trypsin and Trypsin-like Proteolysis.  |  Silzel, JW., et al. 2022. Anal Chem. 94: 15288-15296. PMID: 36279259
10. Trypsin inhibitors.  |  Bhoola, KD. 1970. Lancet. 1: 778. PMID: 4191275
11. Mesotrypsin: a new inhibitor-resistant protease from a zymogen in human pancreatic tissue and fluid.  |  Rinderknecht, H., et al. 1984. Gastroenterology. 86: 681-92. PMID: 6698368
12. Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis.  |  Scheele, G., et al. 1981. Gastroenterology. 80: 461-73. PMID: 6969677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Trypsin Antibody (D-1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-Trypsin Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Trypsin Antibody (D-1) Specifications

Alternate Product Name: PRSS1

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 39-140

Epitope Target: Trypsin-1

Epitope Species: human

Product Reactivity/Detection: Trypsin-1, Trypsin-2 and Trypsin-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for Trypsin Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin