Trx Antibody (D-4): sc-271281

Trx Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Thioredoxin (Trx ) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Trx (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Trx is a crucial redox protein that plays a significant role in cellular processes by maintaining the redox balance within cells. Trx is primarily located in the cytoplasm but can also be found in the nucleus and mitochondria, highlighting versatility in cellular functions. Trx presence in these locations is vital for protecting cells from oxidative stress, as Trx helps to reduce disulfide bonds in proteins, thereby maintaining their functional state. Additionally, Trx is involved in regulating various signaling pathways and transcription factors, which are essential for cell growth and survival. Trx interaction with other proteins, such as Thioredoxin Reductase (TrxR), further emphasizes importance in cellular redox homeostasis and potential implications in diseases, including cancer, where Trx overexpression can contribute to tumor progression and resistance to therapy.

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Trx Antibody (D-4) References:

1. Physiological functions of thioredoxin and thioredoxin reductase.  |  Arnér, ES. and Holmgren, A. 2000. Eur J Biochem. 267: 6102-9. PMID: 11012661
2. Thioredoxin reductase two modes of catalysis have evolved.  |  Williams, CH., et al. 2000. Eur J Biochem. 267: 6110-7. PMID: 11012662
3. Thioredoxin reductase as a pathophysiological factor and drug target.  |  Becker, K., et al. 2000. Eur J Biochem. 267: 6118-25. PMID: 11012663
4. Redox regulation by thioredoxin superfamily; protection against oxidative stress and aging.  |  Tanaka, T., et al. 2000. Free Radic Res. 33: 851-5. PMID: 11237106
5. Properties and biological activities of thioredoxins.  |  Powis, G. and Montfort, WR. 2001. Annu Rev Pharmacol Toxicol. 41: 261-95. PMID: 11264458
6. The thioredoxin (Trx) redox state sensor protein can visualize Trx activities in the light/dark response in chloroplasts.  |  Sugiura, K., et al. 2019. J Biol Chem. 294: 12091-12098. PMID: 31217277
7. Enhanced Oxidative DNA-Damage in Peritoneal Dialysis Patients via the TXNIP/TRX Axis.  |  Oberacker, T., et al. 2022. Antioxidants (Basel). 11: PMID: 35740021
8. MiR-199-3p Suppressed Inflammatory Response by Targeting MECP2 to Alleviate TRX-Induced PHN in Mice.  |  Wang, Z., et al. 2022. Cell Transplant. 31: 9636897221108192. PMID: 35838296
9. Thioredoxin.  |  Holmgren, A. 1985. Annu Rev Biochem. 54: 237-71. PMID: 3896121
10. Amplification and purification of plasmid-encoded thioredoxin from Escherichia coli K12.  |  Lunn, CA., et al. 1984. J Biol Chem. 259: 10469-74. PMID: 6381486
11. Localization of thioredoxin from Escherichia coli in an osmotically sensitive compartment.  |  Lunn, CA. and Pigiet, VP. 1982. J Biol Chem. 257: 11424-30. PMID: 6811581
12. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.  |  LaVallie, ER., et al. 1993. Biotechnology (N Y). 11: 187-93. PMID: 7763371

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Trx Antibody (D-4) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Trx Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Trx Antibody (D-4) Specifications

Alternate Product Name: Trx

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-34

Epitope Target: Trx

Epitope Species: human

Product Reactivity/Detection: Trx

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Trx Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin