Troponin T-SS CRISPR/Cas9 KO Plasmid (m): sc-423460
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Troponin T-SS CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Troponin T-SS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Troponin T-SS Antibody (A-9): sc-365014
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Troponin T-SS CRISPR/Cas9 KO Plasmid (m) | sc-423460 | 20 µg | $397.00 |
Overview
Tnnt1 encodes slow skeletal muscle troponin T (Troponin T-SS), a core component of the thin filament troponin complex that couples Ca²⁺ binding by troponin C to tropomyosin movement and actin–myosin cross-bridge cycling. By regulating sarcomere contraction kinetics and Ca²⁺ sensitivity, Troponin T-SS helps define slow-twitch fiber function and contributes to muscle development and adaptive remodeling. Altered TNNT1 expression or sequence is linked to inherited myopathies and contractile dysfunction, making it relevant for studies of sarcomeric integrity, excitation–contraction coupling, and fiber-type specification. In mouse systems, Tnnt1 perturbation supports mechanistic interrogation of muscle weakness phenotypes and downstream transcriptional and proteostatic responses.
Troponin T-SS CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnnt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnnt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnnt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin T-SS protein expression.
This CRISPR knockout system enables efficient generation of Tnnt1-deficient cell models for investigation of Troponin T-SS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnnt1 exon(s) critical for Troponin T-SS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnnt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Troponin T-SS CRISPR/Cas9 KO Plasmid (m) and Troponin T-SS CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnnt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Troponin T-SS HDR Plasmid (m) and Troponin T-SS HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnnt1 homology arms to support homology-directed repair at defined Tnnt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.